human interferon β (inf-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. in this study an expression vector was designed and constructed for expression of human inf-β-1b either in shake flasks and bench top bioreactor. the designed vector was constructed based upon pet-25b(+) with t7 promoter. recombinant human beta interferon ...

tgiflx, a homoproteins cluster member located on the x chromosome, has a critical role in male reproduction and prostate development. previous studies have shown the erratic expression of tgiflx gene in a large proportion of prostate tumors. however tgiflx function in prostate development remains unknown. the purpose of this study was to evaluate the consequences of tgiflx expression on prostat...

conclusions the results of the present study will increase our knowledge about molecular cloning and expression of the l. infantum kmp-11 gene, and this may be used as an effective target for controlling visceral leishmaniasis. results the kmp-11 gene was successfully subcloned in pcdna3 as a eukaryotic expression vector. recombinant kmp-11 protein was confirmed by the reverse transcriptase pol...

Abstract The persistence of plasmids in bacterial populations represents a puzzling evolutionary problem with serious clinical implications due to their role the ongoing antibiotic resistance crisis. Recently, major advancements have been made toward resolving this “plasmid paradox” but mainly nonclinical context. Here, we propose an additional explanation for maintenance multidrug-resistance E...

Klebsiella species occupy a wide range of environmental and animal niches, occasionally cause opportunistic infections that are resistant to multiple antibiotics. In particular, pneumoniae ( Kpne ) has gained notoriety as major nosocomial pathogen, due principally the rise in non-susceptibility carbapenems other beta-lactam Whilst it been proposed urban water cycle facilitates transmission path...