We have previously reported that ectopically expressed factor VIII (FVIII) is stored within platelets and is released upon platelet activation. Studies by others in various cell lines have suggested that having von Willebrand factor (VWF) coexpression is necessary for FVIII granular storage and for its secretion. We tested the importance of VWF coexpression for ectopic storage of FVIII in plate...

INTRODUCTION Leukocyte-platelet rich fibrin belongs to a second generation of platelet concentrates that does not need biochemical blood manipulation. It is used for tissue healing and regeneration in periodontal and oral-maxillofacial surgery. We report two cases of hyperplastic gingival lesions treated by exeresis and application of leukocyte-platelet rich fibrin membranes in order to improve...

We have previously reported that ectopically expressed factor VIII (FVIII) is stored within platelets and is released upon platelet activation. Studies by others in various cell lines have suggested that having von Willebrand factor (VWF) coexpression is necessary for FVIII granular storage and for its secretion. We tested the importance of VWF coexpression for ectopic storage of FVIII in plate...

77 In contrast to the results of the study by Metcalfe et al., Costa et al. did not observe higher levels of P-selectin in PRP PCs than in BC-PC. However, the BC-PC may have been prepared in a different way. The cytokines measured are generally derived from white blood cells. The platelet units used in the present study were not leukocyte-reduced, suggesting that higher levels of leukocyte-deri...

Membranous microparticles (MP) appearing in the supernatant plasma of stored platelet concentrates (PC) were analyzed by flow cytometry. Two populations of MP were arbitrarily delineated by light scatter as larger or smaller than 0.5 micron fluorescent beads. An estimate of MP concentration was obtained by adding a known amount of fluorescent beads to each sample before analysis of a set number...

Background: Red blood cells (RBCs) undergo biochemical and morphologic alterations during storage that are known as the storage lesions causing decreased RBC quality and are correlated with transfusion reactions in certain groups especially in infants and critically ill patients. Microvesicles (MVs) as one of storage lesions may be derived from various cell types and have key roles in several...

irish potatoes is rich in carbohydrate, with varied glucose levels. it is also a very good source of apyrase, an enzyme used for platelet storage. the aim of this study was to explore irish potatoes as an easily available source for apyrase extraction and purification. apyrase was extracted from 5 kg of irish potatoes. platelets were derived from 5-10 ml of human whole blood and g6pd activity w...

i read with interest dr. yazdani's reply about the article entitled “administration of magnesium sulfate to women with premature labor: the effect on bleeding time”. 1 in my review of hematology references, i could not find any reference for determining hemoglobin (hb) sooner than 24 hrs and dr. yazdani had not cited any reference either. however, about the influence of hb on homeostasis, there...