The aim of this study was to evaluate of the quantification of Pneumocystis jiroveci using a real-time PCR assay. We tried to verify whether quantification was really effective in differentiating between carriage and Pneumocystis pneumonia (PCP) using real-time PCR with or without sample species normalization for classifying each sample species (sputum, bronchoalveolar lavage: bronchoalveolar l...

Pneumocystis jiroveci pneumonia (PJP) is a feared opprtunistic infection in the renal transplant population. The unicellular fungus is ubiquitous in the environment but has an untreated mortality of 90–100% in immunocompromised HIV-negative patients [1]. This falls to 35% with treatment [2]. Many, but not all, transplant centres routinely prescribe PJP prophylaxis. Current European Best Practic...