BACKGROUND Oxygen-dependent quenching of luminescence using transparent planar sensor foils was shown to overcome the limitations of the polarographic electrode technique in an animal model. This method was then transferred to a clinical setting to measure the transcutaneous pO(2) (p(tc)O(2)). METHODS In six healthy subjects, a cuff on the upper arm was occluded up to 20 mmHg above systolic p...

A differential-pulse polarographic (DPP) method was developed for the determination of brodimoprim (BDP) in Michaelis (MCL) buffer. The peak potential (Ep) occurs at 21.33 V (versus SCE) when the pH of the system is 4.0. The voltammetric behaviour in MCL buffer and the mechanisms of the electrode reaction were studied by, e.g., linear sweep voltammetry, cyclic voltammetry and normal-pulse polar...

The method is based on the separation of Tl(I) as Tl(2)HPMo(12)O(40), stripping of the molybdate, and measurement of the peak current in differential-pulse polarography of the molybdenum. The calibration graph is linear over the range 2-12 ppm of thallium. The relative standard deviation is 1.2% (7 replicates each containing 500 microg of thallium). The current due to reduction of the molybdenu...

The purpose of this study is to establish a procedure for the direct determination of oxygen tension of whole blood that requires a smaller sample and simpler manipulation than methods previously reported (1). A procedure has been worked out for determining dissolved plasma oxygen which proved satisfactory in our investigations. Since the success of the application of the proposed polarographic...

A highly sensitive differential pulse polarographic method has been developed for the estimation of tegaserod maleate after treating it with hydrogen peroxide solution. The oxidation of tegaserod maleate is a reversible process as the oxidized product could be reduced at hanging mercury drop electrode in a quantitative manner using differential pulse polarography mode. The limit of quantificati...

1. The rate of the saponification of iodoacetic acid in sodium hydroxide and alkaline buffer solutions yielding glycollic acid was measured by means of Heyrovský's polarographic method. 2. From the bimolecular velocity constants, increasing with the ionic strength of the solution, the Brönsted factor, F, which characterizes the primary salt effect, was calculated. 3. In the borate buffer soluti...

The polarographic behaviour of salicylaldehyde-2-pyridylhydrazone (SAPH) has been studied in aqueous buffer solution containing 40% ethanol using DC and DP polarographic methods. In the pH range 1.8--7.5 the observed single irreversible reduction wave is assigned to the splitting of the N N bond and reduction of C = N centre. In alkaline medium, a second wave appears at a more negative potentia...

Polarographic studies have been carried out recently on several carbonyl1 and carhonyl-nitrosyl 2 complexes with the aim of obtaining information on the electronic structure of the M — CO and M — NO bonds. In the reduction of carbonyl complexes the electronic change is localized on a prevailing central metal orbital. In the nitrosyl complexes, however, it is the JTNO 2 orbital which is involved...

Glucose concentration may be determined enzymatically by a glucose-oxidase procedure in which the amount of oxygen depletion from a solution saturated with oxygen is observed. The difference between the initial and final oxygen levels as detected by a polarographic oxygen analyzer is a measure of glucose concentration. Blood is sampled continuously by means of a double-lumen catheter inserted i...