The exploitation of phenolic compounds in different fields has motivated researchers to explore eco-friendly and efficient extraction techniques. This study aimed comparatively reveal that green techniques (microwave- ultrasound-assisted) are alternative conventional (maceration magnetic stirring) with positive impact on the content, antioxidant activity, bioactive profile bee pollen extracts. ...

The aim Evaluation of the applicability of immunoblotting for diagnosing cross reactivity between homologous proteins present in various sources. Method and material: Group of 21 patients with birch pollen allergy aged 41,7±5,2 revealing symptoms after eating cross-reacting food and positive skin tests, or prick by prick tests with native food allergens. Control group 7 persons aged 39,8±4,7, a...

Nicotiana tabacum pollen tubes contain two high molecular weight polypeptides (about 400 kDa), which are specifically expressed during pollen germination and pollen tube growth in BK medium. The high molecular weight doublet resembles the dynein heavy chains in some biochemical properties. Sedimentation profiles of pollen tube extracts show that the high molecular weight bands have sedimentatio...

BACKGROUND European studies provide a preponderance of evidence for sublingual allergen immunotherapy (SLIT) safety and efficacy, but they use allergen products that differ from those expected to be approved in the United States. OBJECTIVE To determine the safety and tolerability of 4 US-licensed standardized SLIT allergenic extracts. METHODS Adults 18 to 50 years old with allergic rhinitis...

abstract background: bee-collected pollen and propolis are apicultural products which are recognized as a well balanced food. these beehive products are composed of nutritionally valuable substances and contain considerable amounts of polyphenol substances which may have several useful pharmacological properties. methods: the protective activity of bee-collected pollen (bpe) and water-soluble d...

SHY, a pollen-specific gene identified in a screen for genes upregulated at pollen germination, encodes a leucine-rich repeat (LRR) protein that is predicted to be secreted. To test if SHY plays an important role during pollen germination, we generated transgenic plants expressing an antisense (AS) copy of the SHY cDNA in pollen. Primary transformants exhibited poor seed set, but homozygous lin...

Lung surfactant protein A (SP-A) is the most abundant surfactant-associated protein present in the lung. A receptor for SP-A has been shown to be present on A549 alveolar type II cells and on other cell types, including alveolar macrophage. The SP-A receptor on A549 cells has been identified as the collection receptor, or C1q receptor, which binds several structurally-related ligands. SP-A cont...

BACKGROUND Paper mulberry (Morus papyrifera) pollen is considered to be one of the most clinically relevant aeroallergens in Pakistan. To date, the allergenicity of the pollen has not been investigated. OBJECTIVE To characterize the sensitization profile of mulberry-allergic patients and the proteins of paper mulberry pollen contributing to pollinosis in the Pakistani population. METHODS Pr...

BACKGROUND The pollen coat is the first structure of the pollen to encounter the mucosal immune system upon inhalation. Prior characterizations of pollen allergens have focused on water-soluble, cytoplasmic proteins, but have overlooked much of the extracellular pollen coat. Due to washing with organic solvents when prepared, these pollen coat proteins are typically absent from commercial stand...