Abbreviations: 434(1–63) – N-terminal 63-residue DNA-binding domain of the phage 434 repressor; dh434(0–63) – variant 434 repressor DNA-binding domain devoid of hydroxyl groups; P22c2(1–76) – N-terminal 76-residue fragment of the phage P22 c2 repressor; SDS-PAGE – SDS polyacrylamide gel electrophoresis; COSY – correlation spectroscopy; TOCSY – total correlation spectroscopy; NOE – nuclear Overh...

Proteins labeled with a luminescent lanthanide chelate, {{2,2',2'',2'''-{4'-{[(4,6-dichloro-1,3,5-triazin-2-yl)amino]biphenyl-4-yl}-2,2':6',2''-terpyridine-6,6''-diyl}bis(methylenenitrilo)}tetrakis(acetato)}europium(III) (DTBTA-Eu(3+)),were analyzed with sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) slab gel electrophoresis and hydroxyethyl cellurose gel capillary electrophoresis with a ...

DNA mobility shift assay is widely performed to analyze sequence-specific DNA-binding proteins in cellular extracts (2), and the DNA-binding proteins can be identified by use of antibodies that induce further retardation of probes (supershift). Gel extracts from a shifted band contain not only DNA segments and DNA-binding proteins but also nonspecific proteins located at the same position in th...

Currently, there is an interest in novel drug delivery systems, diagnostic capabilities and improving separation of biomacromolecules such as DNA and proteins. One possible approach is to add charged nanoparticles to the polyacrylamide gel electrophoresis system to observe the difference in, for example, protein separation efficiency. Another approach included creating templated pores by polyme...

A new cholesterol-binding protein (CBP) has been purified from rat liver cytosol by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. It appears to be homogeneous by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis, and, using antiserum to purified CBP, by Ouchterlony double-immunodiffusion analysis, by immunoelectrophoresis, and by SDS gel electroph...

Objective: The aim of this study was to evaluate the protein digestibility and analyze the residual enzymatic activity of lysozyme. Methods: Protein digestibility was evaluated hydrolyzing the protein with pepsin at pH 1.2, 2.0, and 3.2 during 60, 90, and 120 minutes of incubation. These hydrolysates were analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reversed-phase...

Solubilization at 75 degrees C of Rhodopseudomonas sphaeroides chromatophores in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (beta-ME) resulted in the selective absence of reaction center B and C polypeptides from SDS-polyacrylamide gel electrophoresis profiles. A newly identified, chromatophore-specific polypeptide, with a mass of 35.2 kdaltons, was also missing under th...

The novel a-amylase-pullulanase produced by Clostridium thermohydrosulfuricum E 101-69 was purified as two forms (I and II) from culture medium, by using gel filtration in 6 M-guanidine hydrochloride as the final step. Renatured a-amylase-pullulanase I and II had apparent Mr values of 370000+85000 and 330000+85000 respectively, as determined by native polyacrylamide-gradient-gel electrophoresis...

Advanced BioMatrix offers a large number of extracellular matrix (ECM) and three dimensional (3D) scaffold products with one of the most extensive Type I collagen product offerings (see Exhibit 1). One analytical method for evaluating the quality, purity and consistency of the products is Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS PAGE electrophoresis). The purpose of the do...