abstract        background and objective: chabahar is in southern iran located near the iran-pakistan border. since leishmaniasis is an emerging disease in this region, this study aimed to diagnose the disease and identify different species of leishmania parasite in the patients referred to the central laboratory.       methods: this descriptive cross-sectional study was conducted in 2011-2012 ...

background and objective: the outcome of hepatitis b virus (hbv) infection may be influenced by host factors like human leukocyte antigen (hla). we have investigated hla-a and drb1 alleles in patients with persistent hepatitis b infection compared to subjects who had spontaneously recovered from hbv infection. to complete the findings of this study we performed another survey in certain hla all...

background: toxoplasmosis is one of the most important food-borne diseases distributed worldwide. in order to determinate the involvement of poultry meat in clinical toxoplasmosis, this study aimed to genetic characterization of toxoplasma gondii in tissue samples of serologically positive chickens of tunisia using polymerase chain reaction (pcr). methods: trail of isolation of local t. gondii ...

background: cytochrome p450 2c19 (cyp2c19) is important in metabolism of wide range of drugs. cyp2c19*17 is a novel variant allele which increases gene transcription and therefore results in ultra-rapid metabolizer phenotype (urm). distribution of this variant allele has not been well studied worldwide. the aim of present study was to investigate allele and genotype frequencies of cyp2c19*17 in...

conclusions disseminated bcg infection should be considered in the differential diagnosis of infants and young children with fever, hepatosplenomegaly, lymphadenopathy, and history of bcg vaccination. the pcr method has a high positivity rate and can serve as a useful tool for the rapid and specific identification of m. bovis bcg substrain infection. results from the total of 34 children hospit...

results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...

Amplified fragment length polymorphism (AFLP) and enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) genomic fingerprinting assays were compared for their ability to differentiate Escherichia coli isolates obtained from various host sources, and with respect to their pathogenicity. One hundred and ten verotoxigenic, enterotoxigenic and non-pathogenic E. coli is...

introduction and aims: the role of human papillomavirus (hpv) in the etiology of esophageal squamous cell carcinoma (escc) is not clear. previous studies have found highly variable (from 0% to 67%) prevalence of hpv in escc tumor. however, prevalence of hpv in escc tumor seems to be higher in areas with high incidence of escc , such as china and south africa. iran is one of the areas of the wor...

conclusions the observed results indicated the necessity of good care in abattoir, butcheries and during food distribution, mainly ground beef. also, a zero tolerance policy could be useful to control e. coli o157 in meat products nationwide. results overall, 1.5% of ground beef samples were contaminated with the o157 e. coli strain meanwhile 1% of samples contained the o157:h7 strain and 0.5% ...

unknown cellular flanking DNA sequences from foreign integrants. Cell Mol. Life Sci. 54, 1403–1411. 5. Iwahana, H., Tsujisawa, T., Katashima, R., Yoshimoto, K., and Itakura, M. (1994) PCR with end trimming and cassette ligation: A rapid method to clone exon-intron boundaries and a 5 -upstream sequences of genomic DNA based on a cDNA sequence. PCR Methods Appl. 4, 19–25. 6. Jones, D. H., and Win...