conclusions: nested pcr as a sensitive chlamydia trachomatis detection test and endocervical specimens has been offered to detect this bacterium in spontaneous abortion. besides, c. trachomatis screening among pregnant women can be suggested to prevent abortion. results: the number of intercourses per week and history of miscarriage can be known as the risk factors of abortion. frequency of c. ...

background and aims: helicobacter pylori is the primary cause of various diseases. this study was conducted to determine the existence of h. pylori in springs and surface water in chaharmahal and bakhtiari province. methods: in this study, 29 water samples were collected from three different locations of zayanderud river, 24 springs, and two subterranean from june 2014 to august 2014 in chaharm...

conclusions there was a significant correlation between crs and presence of h. pylori in sinonasal mucosa. this relationship may reflect the role of h. pylori as one of the pathogenic factors in the development of crs. however, further studies are required to confirm this role. results nine patients (18%) with crs had h. pylori isolated from their samples whereas in the control group, h. pylori...

background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...

background: classical bacteriological detection of brucella species from food, and environment is routinely carried out based on morphological and biochemical characteristics. however, for increasing specificity and sensitivity of species identification methods, development of a molecular assay is necessary that was main aim of this study. methods: panel of some reference strains belonging to t...

background: one of the adulterant practice in dairy industry is the use of a less costly type of milk such as cow and goat milk instead of more expensive ones especially sheep milk. the aim of the present study was to assess fraud identification of undeclared milk species in composition of sheep yogurt and cheese offered as “pure sheep” in iranian local markets. methods: samples of sheep yogurt...

foodborne pathogens comprise microorganisms such as viruses, bacteria and parasites that can be transmitted by food and affect public health worldwide. the most common viruses transmitted via food are hepatitis a virus and norwalk-like caliciviruses. also, the most common bacteria involved in foodborne illnesses are campylobacter jejuni, clostridium perfringens, salmonella spp, escherichia coli...

purpose: to compare the value of conventional laboratory methods and polymerase chain reaction (pcr) in the diagnosis of fungal keratitis. methods: this cross sectional study was conducted at khalili hospital, shiraz, iran. samples were taken from thirty-eight patients with findings suspicious for fungal keratitis. corneal scrapings were used for gram, giemsa and koh stains, culture and pcr ana...

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.