Capsules from a range of pathogenic bacteria are the key determinants of virulency. The capsule hasbeen implicated in virulence of Pasteurella multocida. In this study a type-specific polymerase chain reaction(PCR) assay was used for capsular typing of 39 avian P. multocida isolates from Iran. The PCR amplified afragment of 1044 bp from all of tested isolates. It was found that all avian P. mul...

Background: Formation of secondary structure such as DNA hairpins or loops may influence molecular genetics methods and PCR based approaches necessary for genetic engineering, in addition to gene regulation. Materials and Methods: A polymerase chain reaction with splice overlap extension (SOE-PCR) was used to create fully synthetic 1F5 chimeric anti-CD20 heavy- and light-chain genes. The chi...

thirtyfrieswal lactating cows were screened for clinical and subclinical mastitis and subsequently classified into healthy, subclinically affected and clinically affected groups each group comprising of 10 cows. polymorphism of cow lactoferrin (ltf) gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). the results showed that lft gene pro...

BACKGROUND AND OBJECTIVES Pyrococcus woesei is a hyperthermophilic archaea and produces a heat stable polymerase (Pwo polymerase) that has proofreading activity. MATERIALS AND METHODS In this study, this microorganism was cultured, its DNA was extracted and the pwo gene polymerase was cloned, expressed and purified. The DNA sequence of the cloned gene was verified by sequencing. The pwo polym...

Purpose: Fusobacterium nucleatum is an opportunistic pathogen involved in periodontal diseases, extraoral infections, and colorectal cancer. necrophorum causes a variety of necrotic infections. F. are classified into five two subspecies, respectively. Conventional identification methods were technically hard to distinguish each subspecies species accurately. The purpose the present study was de...

The thermostable properties of the DNA polymerase activity from Thermus aquaticus (Taq) have contributed greatly to the yield, specificity, automation and utility of the polymerase chain reaction method for amplifying DNA (1). We report here the cloning and nucleotide sequence of a DNA polymerase gene from another thermophilic bacterium — Thermus flavus (Tfl). The cloning of the DNA polymerase ...