Siberian wildrye (Elymus sibiricus L.) is a perennial, self-fertilizing grass that plays an important role in animal husbandry and environmental sustenance. However, the transcriptomic and genomic information on this species is very limited, which hinders genetic and breeding studies. In the present study, 76,686,804 clean reads were generated from 11 different tissue samples of E. sibiricus by...

DNA fingerprinting has become an important tool for diversity assessment and varietal identification in plant breeding programs. Semi- random PCR primers targeting intron-exon splice junctions (ISJ) were used to evaluate the potential of these markers in identification and classification of rice genotypes. A total of 12 ISJ primers were used for screening fourteen Egyptian rice genotypes, inclu...

to evaluate the genetic diversity of native populations of crown imperial (fritillaria imperialis l.) in zagross region, issr molecular along with 32 morphological traits were employed. a number of 160 samples corresponding to eight zagross populations distributed in six provinces were collected and assessed. using six primers, 57 bands were totally produced from which 49 were polymorphic. mean...

Genetic diversity estimation of plant materials is one of the important pre-breeding activities in breeding field crops. Twenty-one microsatellite markers used to assess genetic diversity and relationship of 68 sunflower genotypes (Helianthus annuus L.). All of 21 pairs of SSR (Simple Sequence Repeats) markers produced a total number of 49 polymorphic bands. DNA fragments ranged from 9...

Ten polymorphic microsatellite loci for the obligate biotrophic, oomycete pathogen of tobacco, Peronospora tabacina, were identified from a small insert genomic library enriched for GT motifs. Eighty-five percent of the 162 loci identified were composed of dinucleotide repeats, whereas only 4% and 11% were tri-and tetra-nucleotide repeats respectively. About 82% of all the microsatellites were ...

Current databases of Phyllostachys pubescens full-length cDNAs (FL-cDNAs) provide a rich source of sequences for the development of potential FL-cDNA simple sequence repeat (SSR) markers. We screened 10,608 P. pubescens cDNAs, discovering 1614 SSRs in 1382 SSR-containing FL-cDNAs. The SSRs were more abundant within transposable elements (TEs) than expressed sequence tags (ESTs) and genome surve...

To identify cultivars quickly and accurately, DNA fingerprinting of 10 broccoli varieties was performed by using simple sequence repeat (SSR) marker technology. Highly informative polymorphic SSR markers were screened rapeseed. Out the 93 pairs, 21 pairs selected found to have good polymorphism. Each pair generated 1 bands with an average 4.29. The polymorphism information content (PIC) 0.41 a ...

To improve quantitative traits, it is essential to acknowledge genetic structure and diversity of the crop plants. In this study, 54 barley cultivars released from 1963 date by different institutes in both Turkey Bulgaria were screened with 18 iPBS four SCoT markers evaluate population diversity. According results, while total allele numbers was identified as 560, polymorphic ones found 530 (43...

the association between 140 microsatellite bands of 20 primer pairs and 13 quantitative traits recorded on 70 aegilops crassa from iran was evaluated. pearson’s coefficients showed significant correlations between some morphological traits. polymorphic information contents (pic) of loci were in the range of 0/16 (xgwm190) to 0/44 (xgwm161). the result of multiple regression analysis showed sign...

one of the traits of the greatest financial impact on sheep production is the number of lambs weaned per ewe with fecb considered as the most well known major gene concerned with this trait. in order to conduct a marker assisted introgression (mai) program the presence of fecb responsible mutation in afshari sheep breeding flock was surveyed. direct test to detect fecbb allele was conducted on ...