Seed dormancy and resistance to decay are fundamental survival strategies, which allow a population of seeds to germinate over long periods of time. Seeds have physical, chemical, and biological defense mechanisms that protect their food reserves from decay-inducing organisms and herbivores. Here, we hypothesize that seeds also possess enzyme-based biochemical defenses, based on induction of th...

This paper reports the inhibition kinetics of mushroom (Agaricus bisporus; J.E. Lange) polyphenol oxidase (PPO) with benzoic acid, sodium azide and sodium fluoride as inhibitors, and pyrocatechol as substrate. Mushroom PPO was significantly inhibited by all of the tested inhibitors. Diphenolase activity of mushroom PPO exhibited the competitive-type inhibition with benzoic acid as an inhibitor;...

Bruce's Sport is a mutant grapevine (Vitis vinifera L.) with green and white variegated fruit derived from the Sultana variety. The white regions of tissue have decreased polyphenol oxidase (PPO) activity resulting in a reduced capacity for browning. Active PPO from Sultana grapes was purified and had an apparent molecular weight of 40,000 on sodium dodecyl sulfate-polyacrylamide gel electropho...

Banana blossoms are rich in fiber and nutrients a popular plant-based, vegan alternative to fish. However undesirable browning, usually visible at the peduncle cut-end, negatively impacts consumer acceptability of banana blossoms. The aim this work was develop safe alternatives prevent browning First, activities primary enzymes associated with tissue i.e., polyphenol oxidase (PPO), peroxidase (...

Abstract Nitric oxide (NO) is a highly reactive gaseous small molecule that regulates plant growth. Sodium nitroprusside (SNP) was used as an NO donor to study the effects of treatment on browning and antioxidant activity button mushrooms ( Agaricus bisporus ). The findings revealed exogenous alleviated browning, decreased hardness, increased weight loss stored at 4°C for 15 days, reduced accum...

polyphenol oxidase (ppo), also known as tyrosinase (ec 1.14.18.1), is a copper-containing enzyme widely distributed in microorganisms, animals, and plants. this enzyme is responsible not only for browning in plants but also for melanization in animals. thus, tyrosinase inhibitors have a huge impact on industry and the economy. in the current study, we first purified the enzyme, then evaluated i...

Inactivation of polyphenol oxidase (PPO) in fresh apple juice was studied moderate electric field (MEF) treatments at strengths the range 85–110 V/cm. The subjected to periodic on-off 50 °C and constant exposure MEF 65 using two different electro-processing devices, specially constructed for purpose. PPO inactivation by fields determined with respect corresponding control (nofield) treatments. ...

In order to find a way to induce rooting on cuttings of Hemarthria compressa cv. Ya'an under controlled conditions, a project was carried out to study the effect of naphthalene acetic acid (NAA) on rooting in stem cuttings and related physiological changes during the rooting process of Hemarthria compressa. The cuttings were treated with five concentrations of NAA (0, 100, 200 300, 400 mg/l) at...