Pyrraline is one of the advanced glycation end products formed under non-enzymatic and non-oxidative conditions in vivo. In this study, we developed a novel method for determination of urinary pyrraline using solid-phase extraction as a pretreatment procedure prior to determination by high performance liquid chromatography (HPLC). The Oasis HLB solid-phase extraction cartridge was used for pret...

The chemical composition of grape berries is influenced by various environmental conditions often considered to be representative of a "terroir". If grapes from a given terroir are assumed to reflect this origin in their chemical compositions, the corresponding wine should also reflect it. The aim of this work was therefore to reveal the "terroir" expression within the chemodiversity of grapes ...

Interest in metal complexes in modern inorganic chemistry has resulted in increasing demands for the analysis of these compounds. This paper reviews the most recent applications of high-performance liquid chromatography (HPLC) to the analysis of metal complexes. The review centres on the use of the technique in metal complex syntheses, reactions, characterizations and complexations and retentio...

In the present study we used chiral high performance liquid chromatography and gas chromatography to determine the stereoisomers of racemic alpha-tocopherol. The Chiralcel OD-H and Silar-100 AT columns were used. Statistical evaluation of the results established the precision of these methods.

A new high-performance liquid chromatography (HPLC) method has been developed to detect ultra-low concentrations of bisphenol-A (BPA) (below 1 ng/L (ppt)) using column switching electrochemical detection (ECD). The results were superior to those obtained from manual pretreatment procedure with membrane stationary phase. BPA is inherently ubiquitous in the environment, including tools and solven...

Chromatographic procedures for the separation and identification of actinomycins have been reviewed1}. Only one report of HPLC has appeared2); this described reversed-phase separations of the C complex and of a biosynthetic mixture of actinomycin D (AMD)and congeners containing c/s-4-chloroproline in place of one or both prolines. In the present study a wider range of naturally occurring actino...

An improved high-performance liquid-chromatographic (HPLC) assay for the simultaneous determination of serotonin (5-HT) and 5-hydroxyindole-3-acetic acid (5-HIAA) in human urine is reported. Following the automated precolumn derivatization of urinary 5-HT and 5-HIAA with benzylamine, the derivatives are separated by isocratic elution on a reversed-phase C18 semi-microbore column, and are fluoro...

The measurement of glycosylated haemoglobin in blood is vital for monitoring metabolic control in diabetics [1-3]. The development of many different methods of measurement (based on cation-exchange chromatography, electrophoresis, high performance liquid chromatography [HPLC], affinity chromatography, radio and colorimetric immuno techniques) proves the importance of this assay [4]. Since the f...

N,N'-Methylenebisacrylamide (MBAA), which is an important raw material in a widely industrial area, was synthesized using convenient catalysts. The synthesized monomer was fully characterized by the elemental analysis, fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), 1H-NMR, 13C-NMR, GC/MS, and high performance liquid chromatography (HPLC) analysis. Six differe...

An analytical method based on high-performance liquid chromatography-photodiode array detection was developed for the simultaneous determination of three anti-proliferative withanolides [withalongolide A ( 1: ), withaferin A ( 2: ) and withalongolide B ( 3: )] present in the aboveground biomass of the long-leaf groundcherry, Physalis longifolia. This method was achieved by biomass extraction fo...