background: stevia rebaudiana bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. objective: the present study was conducted to optimize a protocol for rapid micropropagation of stevia rebaudiana by shoot tip explants and to invest...

objective(s): t-cell acute lymphoblastic leukemia (t-all) is an aggressive hematologic malignant tumor. administration of chemical compounds influencing apoptosis and t cell development has been discussed as promising novel therapeutic strategies. valproic acid (vpa) as a recently emerged anti-neoplastic histone deacetylase (hdac) inhibitor and pioglitazone (pgz) as a high-affinity peroxisome p...

background: the health risks of crack cocaine smoking on the oral mucosa has not been widely researched and documented. objective: the purpose of this study was to analyze the proliferative activity of oral epithelial cells exposed to crack cocaine smoke using silver nucleolar organizer region (agnor) staining. methods: oral smears were collected from clinically normal-appearing buccal mucosa b...

introduction: cardiac glycosides such as digoxin or digitoxin are the natural products that are traditionally used to increase cardiac contractile force in patients with heart failure and cardiac arrhythmias. it has been shown that digoxin can directly inhibit the cell proliferation and lead to cell apoptosis. present study was conducted to analyze the effect of digoxin in the cohorts of mesenc...

silybum marianum, is known to have anti-inflammatory, hepatoprotective and anticarciogenic effects. the aim of this study was to compare effects of silymarin, rapamycin and fk506 on proliferation and apoptosis of human t cells stimulated with con a. peripheral blood mononuclear cells (pbmc) were stimulated with concavalin a  ( con a) (5µg/ml) and then treated with different inhibitors (silymari...

a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using l...

Micrografting can be used to produce healthy seedlings, assess scion-rootstock compatibility and promote precocity. The first objective of this project was to study the impacts of in vitro micropropagation of four major Iranian pistachio cultivars, namely ‘Ahmad Aghaei’, ‘Akbari’, ‘Badami Sefid’ and ‘Kalle Ghochi’ in two different medium Murashige and Skoog (MS) and Driver and Kuniyuki...

Background: Multiple myeloma (MM) is a malignant plasma cell proliferative disorder with limited immunotherapy treatment because of T cell dysfunction. Objective: To investigate the immunomodulatory function of bone marrow mesenchymal stromal cells (MM-BMSCs) on CD8+ T cells. Methods: Proliferation and cytotoxicity were detected by c...

objective: cryopreservation of immature testicular tissue should be considered as an important factor for fertility preservation in young boys with cancer. the objective of this study is to investigate whether immature testicular tissue of mice can be successfully cryopreserved using a simple vitrification procedure to maintain testicular cell viability, proliferation, and differentiation capac...