1. A method is described for the primary culture of human breast tumour cells on feeder layers of the STO mouse embryo fibroblast cell line. 2. The secretion of the prostaglandins E2 and F2 alpha from the cells was measured and the results indicate that the secretion of both prostaglandins was dependent on oestrogen-receptor status, with cells from oestrogen-receptor-positive tumours secreting ...

To investigate the components of the prostaglandin biosynthetic pathway, the characteristics of cyclooxygenase (COX)-1, COX-2, hematopoietic prostaglandin D synthase (hPGDS), microsomal prostaglandin E synthase (mPGES), and thromboxane synthase (TXS) were examined for autopsied cases with small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). Autopsied patients (n=120) with lung ...

AKR mice develop spontaneous lymphoid leukemia late (8 to 12 months) in life, although persistent murine leukemia virus production occurs throughout their life. This suggests that age-related changes are involved in development of leukemia. Prostaglandin biosynthesis was therefore studied in 24-hr cultures in vitro at 37 degrees of peritoneal macrophages, splenocytes, thymocytes, bone marrow, a...

PURPOSE We aimed to describe the first case of macular edema following intracorporeal injection of alprostadil, a prostaglandin E1. METHODS This was a retrospective case report followed with optical coherence tomography, fundus photos, and fluorescein angiography images. RESULTS A patient developed bilateral cystoid macular edema following intracorporeal injection of alprostadil, a prostagl...

Cooxidation of xenobiotics may occur during prostaglandin biosynthesis. The ability of prostaglandin endoperoxide synthetase to cooxidize several aromatic amines and other chemicals to mutagenic products was tested with the standard Salmonella tester strains. The microsomal fraction of ram seminal vesicles, a rich source of prostaglandin endoperoxide synthetase, in the presence of the prostagla...

The concentration (10(-8)-10(-5) M)-dependent contractile response of norepinephrine was completely inhibited by cyclooxygenase inhibitors but not by a thromboxane synthetase inhibitor. The amount of prostaglandin F2 alpha was significantly increased in the presence of norepinephrine (10(-5) M). Norepinephrine increased the amount of prostaglandin D2, although not significantly. The norepinephr...

Homogenates of pigeon heart, brain, lung, liver, and the formed elements of blood and of monkey brain, liver, spleen, kidney, lung, uterus, heart, and the formed elements of blood contain an enzyme which catalyzes the reduction of the 9-keto group of prostaglandin E to form prostaglandin F. The prostaglandin E 9-ketoreductase in all of these tissues uses NADPH much more effectively than NADH an...

Prostaglandin F(2alpha) is synthesized by prostaglandin F synthase, which exists in two types, prostaglandin F synthase I (PGFS I) and prostaglandin F synthase II (PGFS II). Prostaglandin F(2alpha) binds to its specific receptor, FP. Our previous immunohistochemical study showed the distinct localization of prostaglandin F synthases in rat spinal cord. PGFS I exists in neuronal somata and dendr...

Orczyk & Behrman (1972) reported experiments in rats that indicated that prostaglandins, acting centrally, may have a role in regulating lutropin secretion from the pituitary. They found that systemically administered indomethacin [I-(Cchlorobenzoyl)-5-methoxy-2-methylindol-3-ylacetic a id] blocked ovulation, and this blockade could be overcome by administering lutropin. Later it was found that...

In man, administration of prostaglandin E, or F, will stimulate labour and abortion (Embrey, 1971; Thiery, 1979), whereas administration of prostaglandin synthase inhibitors will delay delivery and suppress pre-term labour (Wiqvist, 1979). Measurements of prostaglandins E, and F,, in peripheral plasma are hampered by the low concentrations present (Samuelsson, 1973) due to rapid clearance by th...