Human immunodeficiency virus type 1 encodes a protease whose activity is required for the production of infectious virus. An Escherichia coli expression and processing assay system was used to screen 285 protease mutants for temperature-sensitive activity. Fourteen protease mutants had a temperature-sensitive phenotype, and approximately half resulted from conservative amino acid substitutions....

.lead concentration has recently increased in country side fields and industrial regions. in the research, the effect of different concentrations of lead acetate on arthrobotrys oligospora growth, sporulation, trap formation and efficiency and also the activity of the extracellular protease of the fungus investigated. a. oligospora is one of the most important biocontrol agents of plant parasit...

we assessed a collection of 47 fluorescent pseudomonas spp., some with known biological     control activity against certain soil-borne phytopathogenic fungi such as, macrophomina phaseolina, rhizoctonia solani, phytophthora nicotianae var. parasitica, pythium sp. and  fusarium sp. in vitro and the potential to produce known secondary metabolites such as, siderophore, hcn and protease. the resu...

probable use of chemical and biochemical detergents to wash polysulphone membranes using in milk ultrafiltration needs a multidisciplinary investigation. by increasing use of these membranes in food processing, washing and recovery of initial permeate flux, in order to use for a longer time from this technology, has much importance. in this research, influence of chemical detergents including e...

in order to optimization of protein hydrolysis condition (including enzyme to substrate ratio (e/s), temperature and time) for achieving the highest antioxidant activity, the green tiger prawn (p. semisulcatus) mince was tested via commercial protease (alcalase® 2.4l) based on the response surface designed method in 18 treatments and then dried to obtain powder in freeze dryer. the antioxidant ...

The severe acute respiratory syndrome (SARS) coronavirus (CoV) main protease represents an attractive target for the development of novel anti-SARS agents. The tertiary structure of the protease consists of two distinct folds. One is the N-terminal chymotrypsin-like fold that consists of two structural domains and constitutes the catalytic machinery; the other is the C-terminal helical domain, ...

in the present work, a new branch of biotechnological advantage of the latex of an endemic perennial plant, euphorbia microsciadia has been introduced. a novel cysteine protease, designated as microsciadin, was purified from the latex of euphorbia microsciadia by a combination of sequential usage of sp-sepharose fast flow column in two different phs and a final gel filtration chromatography. mi...

actinomycetes are an uncommon agent of human infections and its pathogenic factors are not known. the present study reports a rare case isolation of an actinomycete from a woman pleural fluid; the strain was identified by 16s rrna gene sequence analysis. this strain was tested to produce an extracellular protease that hydrolysis gelatin, casein and hemoglobin on agar mediums. the purification o...

The extracellular protease activity of Aspergillus niger AB4.1[pgpdAGLAGFP]#11, a recombinant strain producing a glucoamylase (GLA)–green fluorescent protein (GFP) fusion protein, was investigated in a 15 l stirred tank reactor and accordingly a pH control strategy was designed to minimize protease activity and increase recombinant yield. By maintaining pH at 6 recombinant protein production wa...

Several phytochemicals exhibited high level of antiviral activity. Medicinal plant possessed activity via many mechanisms included inhibition viral replication, the assembly intracellular infectious virus particles, infectivity, RNA polymerase, DNA neuraminidase, protease, reverse transcriptase and protein expression other mechanisms. The current review discuss medicinal plants with their action.