Bacillus strains isolated from the salteren pond (Kakinada) were screened and identified for high alkaline protease activity. The isolates which were positive on skim milk agar (1%) were selected as protease producing strains. Of the ten bacterial isolates screened, isolate S-8 was observed as a potential haloalkaline protease producer and it was identified as Bacillus cereus strain S8 (MTCC NO...

Proteases mediate important crucial functions in parasitic diseases, and their characterization contributes to the understanding of host-parasite interaction. A serine protease was purified about 43-fold with a total recovery of 60% from a detergent-soluble extract of promastigotes of Leishmania amazonensis. The purification procedures included aprotinin-agarose affinity chromatography and gel ...

hydrolysis. Protease was purified 285-fold from thyroid supernatant fraction by DEAWCellulose and Sephadex G-200 chromatography. The protein sedimented as a single component in the analytical centrifuge with a sedimentation constant of 3.6 S. Enzyme activity of the purified enzyme was increased by mercaptoethanol and cysteine, and inhibited by n-chloromercuribenzoate. A specific antibody:agains...

The production of thermostable extracellular protease by Bacillus stearothermophilus F1 was influenced by the composition of culture medium. Peptone iv derived from soybean was the best organic compound for enzyme production. Sodium nitrate, ammonium salts and amino acids as sole nitrogen sources interfered with protease formation. The addition of most carbon sources to the peptone medium faile...

A protease isolated from Pseudomonas aeruginosa PD100 could act in the presence of SDS and Tween 80. This protease could be useful for degradation of protein in the presence of solvent, dehairing of cow skin and degradation of natural proteins. The immobilized protease showed 15-20% increases in temperature stability and the entrapped enzyme retained 83% of its initial activity after six cycles...

This study describes the characterization of 80 kDa protease showing gelationlytic property among three proteases in the excretory/secretory proteins (ESP) from Toxoplasma gondii. The protease activity was detected in the ESP but not in the somatic extract of RH tachyzoites. This protease was active only in the presence of calcium ion but not other divalent cationic ions such as Cu(2+), Zn(2+),...

The stability and reusability of soluble enzymes are of major concerns, which limit their industrial applications. Herein, alkaline protease from Bacillus sp. NPST-AK15 was immobilized onto hollow core-mesoporous shell silica (HCMSS) nanospheres. Subsequently, the properties of immobilized proteases were evaluated. Non-, ethane- and amino-functionalized HCMSS nanospheres were synthesized and ch...

Bacillus species is a potent strain which produced the alkaline protease and it is further mutated to yield more amount of enzyme. An alkaline protease producing strain of Bacillus species was isolated from soil. The isolates were purified and screened for the production of protease enzyme. The conditions for enzyme production were optimized under solid substrate fermentation (SSF) and submerge...

The study is aimed to develop a relevant synbiotic promote growth performance of the whiteleg shrimp, Litopenaeus vannamei. For this, four common natural fiber extracts from Arcera banana, Siamese yellow sweet potato, and white potato were screened for supporting Lactobacillus sp. which was isolated shrimp intestines with probiotic activity, prebiotic score, ability induce bacterial enzyme acti...

Investigation on recovery of alkaline protease from B. licheniformis ATCC 21424 fermented wastewater sludge was carried out by centrifugation and ultrafiltration. Optimization of ultrafiltration parameters (transmembrane pressure (TMP) and feed flux) was carried out with 10 kDa membrane. TMP of 90 kPa and feed flux of 714 L/h/m(2) gave highest recovery (83%) of the enzyme from the centrifuged s...