It is possible to use transgenic plants, as bioreactors, for the production of recombinant inexpensive chemicals and drug components. Transient gene expression is an appropriate alternative to stable transformation because it allows an inexpensive and rapid method for expression of recombinant proteins in plant tissues. In recent years, plant viral vectors have been increasingly developed as su...

gene expression profiling of ovarian carcinoma tissues has shown an increase of four-fold expression of sort1 gene. sortilin 1 (ntr-3) is a 95-100 kda protein normally expressed in heart, brain, placenta, skeletal muscle, spinal cord, thyroid, and testis. however, its expression has never been reported in normal ovary. here, we report expression of sortilin 1 in ovarian carcinoma tissues both a...

mammalian expression systems, due to their capacity in post-translational modification, are preferred systems for biopharmaceutical protein production. several recombinant protein systems have been introduced to the market, most of which are under clinical development. in spite of significant improvements such as cell line engineering, introducing novel expression methods, gene silencing and pr...

abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

hepatitis b virus (hbv) is considered as a global health concern and hepatitis b surface antigen (hbsag) is the most immunogenic protein of hbv. the purpose of this study was to evaluate the expression of hbsag on the cell surface of human embryonic kidney cell line (hek293t). after transformation of expression vector pcdna/hbsag to e.coli top10f’, plasmid was extracted and digested with bglii....

background bone morphogenetic protein 7 (bmp7) has been suggested to play a protective role against kidney injury in chronic kidney disease. objectives to identify the critical molecular regulators in the early stage of diabetic nephropathy, we studied the expression of bmp7 and 2 important kidney-specific markers, podocin and tamm–horsfall protein (thp). materials and methods a diabetic nephro...

background: this study detected osteopontin (opn) and matrix metalloproteinase-7 (mmp-7) expressions to explore the roles of opn and mmp-7 in the occurrence, progression, and prognosis of nonsmall cell lung cancer (nsclc). materials and methods: a retrospective study was conducted on nsclc tissues ( n = 152; case group) and adjacent nonneoplastic lung parenchyma (adjacent to tumor >5 cm; n = 15...

Background: Vibrio are the main pathogenic bacteria in aquaculture. The flagellin protein C (FlaC) of Vibrio alginolyticus has good immunogenicity and the prospect of potential application in a vaccine. Objectives: We aimed to evaluate the immunogenicity, protective immunity, and prokaryotic expression fermentation of V. alginolyticu...