Actively immunizing fractions of protein nature have been isolated from broth culture filtrates of Pneumococcus Type I by ultrafiltration, precipitation at a definite hydrion concentration, and the separation of a soluble picrate fraction. The method appears to be suitable for the initial purification of this antigen.

Hog kidney Acylase I, an enzyme which hydrolyzes N-acetylL-amino acids, has been purified approximately 40-fold by Greenstein (1). During the course of the purification of hog kidney dialkylfluorophosphatase, it was consistently found that the protein fractions containing DFPasel activity also possessed very high Acylase I activity. Inasmuch as the DFPase and Acylase I activities were found to ...

From among a wide variety of protein purification techniques affinity chromatography has proved to be particularly effective for separation of proteolytic enzymes and their inhibitors. In this article, following a general description of affinity adsorbents used for purification of proteinases, we overview a simple separation procedure for some serine proteinases and their inhibitors by way of a...

Ovalbumin, as the major component of egg-white, is a globular, biocompatible, nontoxic and biodegradable phosphoglyco protein. This protein with the molecular weight of 44.5 kDa, contains 385 residues of amino acids with isoelectric point (pI) of 4.5. Many purification procedures have been reported for egg-white proteins such as gel permeation and anion exchange chromatography. In this study, w...

In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented. This method was applied to confirm the binding of the human recombinant USF1 protein to its putative binding site (E-box) within the human SOX3 protomer. It has been shown that biotinylated DNA attached to streptavidin magnetic particles spec...

Affinity separation using functionalized microfiltration (MF) membranes provides a hydrodynamically favorable and cost-effective alternative to conventional column chromatography for the separation and purification of a specific protein from mixture of proteins. This type of separation in MF membranes is carried out by functionalizing the membrane with a suitable ligand containing specific inte...

background: based on the information no research has been done on the identification and isolation of anaerobic fungi in the baloochi sheep’s rumen in the dry climate up to now. objectives: the purpose of this research was the separation and study of the appearance morphology of anaerobic fungi in the baloochi sheep’s rumen in sistan region. methods: the semi-defined medium environment was used...