CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were ...

  Background :Although transfusion is a common procedure for treating anemia of prematurity, there is no specific protocol for blood transfusion in premature newborns. So in this study we investigate whether application of a strict protocol has any statistically significant effect on reduction of blood transfusion.   Methods : In this study, first group admitted in NICU during 2005 - 2006 and t...

Parents who have a baby with infantile colic experienced a different thought and emotions such as hopelessness, fatigue and impasses. In this study, our living experience as a professional nursing parents' are represented with new relieving modality for infantile colic. Herein, we report a new relieving dietary protocol for a colicky infant with non- Immunoglobulin E – mediated protein induced ...

background: microdose flare-up gnrh agonist and gnrh antagonist have become more popular in the management of poor ovarian responders (por) in recent years; however, the optimal protocol for por patients undergoing in vitro fertilization has still been a challenge. methods: in this observational study design, two hundred forty four poor ovarian responders were retrospectively evaluated for thei...