Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), t...

Embryogenic calli were induced from longitudinally bisected segments of protocorm-like bodies (PLBs) of Cymbidium Twilight Moon ‘Day Light’, a hybrid orchid, on modified Vacin and Went medium [Bot. Gaz. 110 (1949) 605] supplemented with 1-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (TDZ) within 1 mont...

An efficient genetically stable regeneration protocol with increased phytochemical production has been established for Dendrobium nobile, a highly prized orchid for its economic and medicinal importance. Protocorm like bodies (PLBs) were induced from the pseudostem segments using thidiazuron (TDZ; 1.5 mg/l), by-passing the conventional auxin-cytokinin complement approach for plant regeneration....

We investigated the appropriate growth conditions of shoot and root through in vitro propagation of orchid (Dendrobium sonia) using different gelling agents. Protocorm Like Bodies (PLBs) of orchid were initiated earlier in vitro and one month old protocorms were used for this study. Protocorms were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of starche...

five different media with two concentrations and kinds of carbohydrates and peptone treatments were examined for their effectiveness in promoting asymbiotic seed germination of epipactis  veratrifolia. results revealed significant differences between media on seed germination percentage and protocorm growth and fast medium has the best effect on both. carbohydrate treatments had significant eff...

BACKGROUND AND AIMS Species in the holoparasitic plant family Rafflesiaceae exhibit one of the most highly modified vegetative bodies in flowering plants. Apart from the flower shoot and associated bracts, the parasite is a mycelium-like endophyte living inside their grapevine hosts. This study provides a comprehensive treatment of the endophytic vegetative body for all three genera of Rafflesi...

In Indonesia, the agribusiness sector has begun to expand due increase in entrepreneurship of orchid plants. Several genera and species plants are promising be traded as ornamental plants, one which is moon (Phalaenopsis amabilis). However, limited number seeds or produced by conventional propagation methods takes a long time get new tillers. Therefore, tissue culture techniques can used grow r...