Fluorescent reporter proteins based on flavin-binding photosensors were recently developed as a new class of genetically encoded probes characterized by small size and oxygen-independent maturation of fluorescence. Flavin-based fluorescent proteins (FbFPs) address two major limitations associated with existing fluorescent reporters derived from the green fluorescent protein (GFP)-namely, the ov...

introduction: today the prevalence of teeth decays has considerably decreased. related organizations and institutions mention several reasons for it such as improvement of decay diagnostic equipment and tools which are even capable of detecting caries in their initial stages. this resulted in reduction of costs for patients and remarkable increase in teeth life span. there are many methods for ...

Non –fermenting Gram-negative bacilli are a group of heterogeneous, aerobic, nonsporing bacteria. They saprophytes in nature and also found as commensals man other animals. This study aims to isolate identification NFGNB from various clinical specimens find out their significance among the in-patients admitted at Vijayanagar Institute Medical Sciences, Bellary. 110 isolates age groups sexes wer...

Trichoderma asperellum is one of the plant growth-promoting fungi (PGPF) presently marketed as biopesticide, biofertilizer and in soil amendments, due to its ability protect plants, enhance vegetative growth reduce pathogen populations. Keeping this view, here, we assessed efficacy against pathogens medicinal plants vitro field conditions Leaf spot disease Green leaf Tulsi (Ocimum basilicum). W...

Detecting a variety of biological samples accurately and swiftly in an integrated way is great practical significance. Currently, biofluorescent spectrum detection still largely relies on microscopic spectrometers. In this study, we propose integrable method to detect spectrums with designed liquid crystal tunable filter (LCTF), order identify typical such as cells bacteria. Hela labeled red gr...

Quenched autoligation (QUAL) probes are a class of self-reacting nucleic acid probes that give strong fluorescence signal in the presence of fully complementary RNAs and selectivity against single nucleotide differences in solution. Here, we describe experiments designed to test whether QUAL probes can discriminate between bacterial species by the detection of small differences in their 16S rRN...

DehI is a homodimeric haloacid dehalogenase from Pseudomonas putida that contains the most complex 61 Stevedore's protein knot within its folding topology. To examine how DehI attains such an intricate knotted topology we combined far-UV circular dichroism (CD), intrinsic fluorescence spectroscopy and small angle X-ray scattering (SAXS) to investigate its folding mechanism. Equilibrium unfoldin...