DNA from the Streptomyces plasmid pIJ702 was mutagenized in vitro using hydroxylamine and transformed into Streptomyces lividans. One plasmid with temperature-sensitive replication (pMT660) and one plasmid with a temperature-sensitive tyrosinase (pMT661) were isolated. The plasmid pMT661 contains a novel PstI restriction endonuclease site within the tyrosinase gene.

The gene encoding the outer membrane phosphate-selective porin protein P from Pseudomonas aeruginosa was cloned into Escherichia coli. The protein product was expressed and transported to the outer membrane of an E. coli phoE mutant and assembled into functional trimers. Expression of a product of the correct molecular weight was confirmed by sodium dodecyl sulfate-polyacrylamide gel electropho...

Adverse effects of strain persistence and secretion in milk have been encountered with the Brucella melitensis vaccine strain Rev.1. Field isolates obtained from vaccinated animals and from a human resembled the vaccine strain Rev.1 by conventional bacteriological tests. The lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirma...

High levels of insulin and insulin-like growth factors have been associated with increased risk of prostate cancer (1, 2). Insulin regulates and stimulates cell growth, and inhibits apoptosis in cellular models, although mitogenicity seems to occur at supraphysiologic levels. Insulin also inhibits transcription of insulin-like growth factor binding protein-1 and decreases the synthesis of sex h...

The complete sequence of plasmid pEA29 from Erwinia amylovora strain Ea88 consists of 28,185 bp with a 50.2% G+C content. As deletions and insertions were detected in other derivatives of pEA29, its size actually varied from 27.6 to 34.9 kb. Thirteen open reading frames that encoded predicted proteins with similarities to known proteins from other bacteria were identified along with two open re...

SOURCE/DESCRIPTION : A 1.7 kb Eco RI-Taq I fragment was used as a probe. This fraciment was isolated from pUSE 109 containing a 7 kb Eco RI genomic fragment from the human porphobilinogen(PBG) deaminase(EC 4.1.3.8.) gene. (1) POLYMORPHISM : Pst I digests of human genomic DNA were hybridized to the probe. This presented a two ailele polymorphism with bands of 2.2 kb(A1), 1.4 kb(A2) and constant ...

Calf satellite I DNA digested with EcoRI and PstI gives three fragments 643, 621 and 83 bp long. Two of them, the 643 and 621 bp were cloned using pBR 322 vector and analyzed by means of the MspI and HaeIII restriction enzymes.

The present study was aimed to examine the association of bovine follicular stimulating hormone gene polymorphism with sperm quality traits including sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh m...