A genomic-length cDNA clone corresponding to the RNA of dengue virus type 2 (DEN-2) New Guinea C strain (NGC) was constructed in a low copy number vector. The cloned cDNA was stably propagated in Escherichia coil and designated pDVWS501. RNA transcripts produced in vitro from the cDNA using T7 RNA polymerase yielded infectious virus (MON501) upon electroporation into BHK-21 cells. When compared...

INTRODUCTION Bile and gallstones are spilled during 13% to 40% of all laparoscopic cholecystectomies. They can act as a septic focus and cause complications. We present 2 cases of perihepatic abscess formation due to dropped gallstones presenting some years later. Delayed diagnosis resulted in unnecessary investigations and had negative economic consequences. CASE DESCRIPTION In 1 patient a p...

A 35-year-old man presented with progressive exertional dyspnea (3 months) and syncopal attacks (1 week). He had tachypnea, tachycardia and hypotension. Examination revealed a mid-diastolic plop at the apex. Clinical suspicion was a left atrial mass with a ball valve-like mechanism (LA thrombus or LA tumors). Echo and cardiac MRIs with gadolinium enhancement (to differentiate between a tumor an...

It is widely known that there a lot of useful information hidden in big data, leading to new saying "data money." Thus, it prevalent for individuals mine crucial utilization many real-world applications. In the past, studies have considered frequency. Unfortunately, doing so neglects other aspects, such as utility, interest, or risk. sensible discover high-utility itemsets (HUIs) transaction da...

The notion of a free product of pro-finite groups has some important applications in the theory of algebraic number fields (see [3]). In this connection, it is interesting to get some knowledge about the subgroups of such a free product. The aim of this paper is to show a theorem for the open subgroups of a free pro-finite product, which is an analog of Kurosh’s wellknown subgroup theorem for t...

The complexation of redox sensitive elements by humic acid (HA), described through the charge neutralisation model (CNM) has been reviewed in order to have a comprehensive scope. The data acquired in HUMICS program on thorium (IV), and data available in the literature, were reinterpreted according to the CNM, and adapted to uranium (IV), neptunium (IV) and plutonium (IV) through analogy in orde...

PuO2/Calcination temperature/Inhalation/Lung retention/Translocation Wistar rats inhaled PuO2 particles prepared by the calcination of Pu hydroxide at 1150 and 400°C. Lung retention, fecal and urinary excretion, and translocation of Pu were compared between the two calcination temperatures. The clearance of Pu from the lungs was significantly faster in the rats exposed to PuO2 calcined at 400°C...