An easy-to-use tuberculosis skin test is developed with chitin microneedles that deliver purified protein derivative at the correct skin depth and result in a positive test in BCG-immunized guinea pigs.

BACKGROUND The purified protein derivative (PPD) skin test is the only widely used method which detects latent tuberculosis infection (LTBI) and is dependent on a normal T cell function. In rheumatoid arthritis (RA) the T cell function is altered, which may result in an inability to develop an adequate PPD reaction. OBJECTIVES To evaluate the response to PPD in patients with RA and to compare...

AIM The aim of this study is to interpret purified protein derivative (PPD) induration sizes with respect to the number of Bacillus Calmette-Guérin (BCG) scars. METHODS We have considered 1879 school children between the ages of 7 and 14 years from seven primary schools in Kocaeli, Turkey. Children were injected with 5TU 0.1 mL PPD and induration sizes were measured at 72 h. Number of BCG sca...

OBJECTIVE To determine whether implementation of an electronic health record (EHR) would increase the rate of prenatal Human Immunodeficiency Virus (HIV) and purified protein derivative (PPD) testing. METHODS Eligible participants received prenatal care and delivered at term at a single academic institution in March-April 2011, March-April 2012, and March-April 2013. As part of routine prenat...

In testing persons who are negative to weak dilutions of old tuberculin, it is frequently noted that a disproportionately large reaction may occur with the stronger dilutions. The suspicion has been voiced that these reactions to strong concentrations of old tuberculin may be non-specific. The problem is of special importance in testing children prior to BCG administration. Are those who react ...

ONE of the more perplexing features of sarcoidosis is the abnormally high rate of insensitivity to tuberculin injected intradermally. In any large urban community in England very few adults between ages 30 and 65 will be found not reacting to a dose of ioo units (o.ooz mg.) of tuberculin; fewer still will be found in a comparable age-group of ambulant cases of tuberculosis, yet half or more of ...

Vaccination of mice with Mycobacterium bovis culture filtrate proteins (CFP), prepared in a variety of adjuvants (aluminum hydroxide, Quil-A, and dimethyldioctyldecyl ammonium bromide [DDA]), provided significant protection against an aerosol challenge of virulent M. bovis. Additionally, vaccination with CFP in DDA or Quil-A did not sensitize mice to M. bovis purified protein derivative.

A decrease in the reaction to intracutaneous injection of tuberculin or its purified protein derivative is generally accepted as a feature of the disease syndrome sarcoidosis. The phenomenon has aroused considerable interest, and many discussions of the various possible underlying mechanisms have appeared in the literature.""0'11 A few detailed investigations have been conducted, but there is n...

BACKGROUND & OBJECTIVE To estimate the efficacy of BCG in preventing tuberculosis over a 15-year period, and also to assess the impact of infection with nontuberculous environmental mycobacteria in a rural community in Chingleput district in Tamil Nadu in south India. We re-analysed the 15-year follow up data of a large randomized trial conducted earlier. METHODS A double-blind randomized con...