This study investigated whether peripheral nerve damage in patients with leprosy impairs local cellular immune responses, thereby reducing wound healing and leading to chronic skin ulceration. Anesthetic and contralateral sensitive skin sites in 42 patients with leprosy were compared for delayed-type hypersensitivity responses to purified protein derivative (PPD) of tuberculin. Leukocyte recrui...

T-cell proliferative responses were studied in two villages in Gabon with different levels of Loa loa transmission. The first village (Okoumbi) had an annual transmission potential (ATP) of approximately 9,000 infective larvae (L3)/person/year (high transmission village), while the second village (Ndjokaye) had an ATP of approximately 1,000 L3/person/year (low transmission village). Proliferati...

Delayed cutaneous hypersensitivity (DCH) of 12 normal adult subjects to purified protein derivative (PPD) of Mycobacterium tuberculosis, streptococcal streptokinase-streptodornase (SK-SD), and Candida albicans Dermatophytin 0 (DO) was assayed in vivo by skin testing and compared with such in vitro correlates of cellular immunity as lymphocyte transformation (LT) and inhibition of leukocyte migr...

The Non-Tuberculous Mycobacteria (NTM),whose habitat the environment has acquired importance in the last years because of the immunosupressed patients, infected HIV ,and also in develop countries that have managed to eradicated the bovine tuberculosis. Where it has been verified that certain NTM interfere in the diagnosis of tuberculosis when it is applied to the delayed hypersensitivity test w...

Heat-killed Mycobacterium vaccae vaccine was administered in a three-dose intradermal schedule to 10 healthy adult volunteers at 0, 2, and 10 months. Local and systemic side effects were monitored and vaccine site reactions were measured and photographed at visits 2 days, 14 days, and 2 months after each dose. Reactions to skin tests with purified protein derivative (PPD) and Mycobacterium aviu...

background according to several studies, diabetes mellitus has been recognized as one of the main risk factors for pulmonary tuberculosis (ptb). objectives the main purpose of this study was to determine the frequency of smear-positive pulmonary tuberculosis (tb) in patients with diabetes. materials and methods this cross-sectional study was conducted from april 2010 to december 2011 in ali asg...

BACKGROUND The possible association between (tuberculous and nontuberculous) mycobacterial infections and sarcoidosis is still a matter of dispute. Using diagnostic tests for specific T-cell responses, this association can be investigated in an innovative manner. OBJECTIVE To measure the T-cell responsiveness to the purified protein derivative (PPD) antigen in blood and broncho-alveolar lavag...

PURPOSE The purpose of this study was to evaluate Interferon (IFN)-γ and Interleukin(IL)-17 profiles in patients with different clinical presentations of pulmonary tuberculosis (TB) and to compare them with those of tuberculin-negative and tuberculin-reactive healthy controls METHODS Peripheral blood mononuclear cells (PBMCss), isolated from patients (n=52) and controls (n=30), were stimulate...

BACKGROUND In the outpatient use of tuberculin skin testing (purified protein derivative [PPD]), it is at times inconvenient to have a patient revisit for interpretation. Therefore, we assessed patients' ability to self-interpret these test results. METHODS In keeping with prior custom, patients were seen by an experienced nurse, who performed skin testing with PPD intermediate strength as we...

Bacille Calmette-Guérin (BCG) immunization provides variable protection against tuberculosis. Prenatal antigen exposure may have lifelong effects on responses to related antigens and pathogens. We therefore hypothesized that maternal latent Mycobacterium tuberculosis infection (LTBI) influences infant responses to BCG immunization at birth. We measured antibody (n = 53) and cellular (n = 31) re...