in this study, the effect of hydrocarbon and rosin ester resins combination on the physical and mechanical properties of thermoplastic road markings were evaluated. at first, two basic thermoplastic road marking formulations based on hydrocarbon and rosin ester resins were prepared. several samples of the blends of two basic formulations for thermoplastic road marking were characterized and com...

Control of gluconeogenesis from lactate was studied by titrating rat liver cells with lactate and pyruvate in a ratio of 1 O : l in a perifusion system. At different steady states of glucose formation, the concentration of key gluconeogenic intermediates was measured and plotted against gluconeogenic flux (Jglucoee). Complete saturation was observed only in the plot relating JglUcto the extrace...

In a previous study, an Escherichia coli strain lacking the key enzymes (acetate kinase and phosphotransacetylase, ACK-PTA) of the major acetate synthesis pathways reduced acetate accumulation. The ackA-pta mutant strain also exhibits an increased lactate synthesis rate. Metabolic flux analysis suggested that the majority of excessive carbon flux was redirected through the lactate formation pat...

The control of pyruvate dehydrogenase activity by inactivation and activation was studied in intact mitochondria isolated from rabbit heart. Pyruvate dehydrogenase could be completely inactivated by incubating mitochondria with ATP, oligomycin, and NaF. This loss in dehydrogenase activity was correlated with the incorporation of 32P from [gamma-32P]ATP into mitochondrial protein(s) and with a d...

Introduction of ester groups into positions 4 and 5 of 3-iodopyridazines (1,2) by redox decomposition of the oxyhydroperoxide of ethyl pyruvate in a sulfuric acid/toluene two-phase system affords trifunctional pyridazine building blocks (3,4a) in a single step. Iodoarenes and -hetarenes are known as useful synthons for C-C bond formation by palladium-catalyzed cross-coupling reactions. In the p...

1. A method was devised for preparing pig heart pyruvate dehydrogenase free of thiamin pyrophosphate (TPP), permitting studies of the binding of [35S]TPP to pyruvate dehydrogenase and pyruvate dehydrogenase phosphate. The Kd of TPP for pyruvate dehydrogenase was in the range 6.2-8.2 muM, whereas that for pyruvate dehydrogenase phosphate was approximately 15 muM; both forms of the complex contai...

Pyruvate transport across the inner mitochondrial membrane is believed to be a prerequisite for gluconeogenesis in hepatocytes, which is important for the maintenance of normoglycemia during prolonged food deprivation but also contributes to hyperglycemia in diabetes. To determine the requirement for mitochondrial pyruvate import in gluconeogenesis, mice with liver-specific deletion of mitochon...