Background: Rabies virus is a neurotropic virus that causes fatal, but, a preventable disease in mammals. Administration of rabies immunoglobulin (RIG) is essential for the post-exposure of the prophylaxis to prevent the disease. However, replacement of polyclonal RIGs with alternative monoclonal antibodies (MAbs) that are capable of neutralizing rabies virus has been recommend...

A DNA vaccine against rabies (pGQH) was administrated to cats in order to examine different administration routes. Four groups of three cats each were inoculated with pGQH as follows: group A, intramuscularly (IM), 100 microg; group B, intranasally (IN), 100 microg; group C, intradermally into ear pinnae (ID-EP), 100 microg, and group D, IM, 200 microL of phosphate buffer solution (PBS) alone (...

Animals imported from abroad are a cause of rabies outbreaks in many countries. Therefore, serology testing for dogs and cats traveling is an important measure to reduce the incidence rabies. Rabies virus antibodies were measured sera collected 2,367 894 between 2017 2021. A serum sample with value 0.5 IU/mL or higher was considered pass. The overall pass rates 96.4% 98.4% cats. mean neutraliza...

Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipid formulations on rabies neutralizing titers. Plasmid expressing Rabies G protein (CVS strain) was evaluated in vivo for ability to elicit neutralizing titers. pDNA:DMRIE-DOPE formulated a...

Abbreviations aa aminoacid ADCC antibody dependent cell cytotoxicity APC antigen presenting cell CFA complete Freund's adjuvant cpm counts per minute CTL cytotoxic T lymphocytes CVS challenge virus strain EBL European bat lyssavirus ERA Evelyn-Rokitmicki-Abelseth ERIG equine rabies immunoglobulin FITC fetal calf serum FITC fluoresceine isothiocyanate FFU fluorescent focus units G glycoprotein G...

BACKGROUND The potential role of oral vaccination of dogs against rabies in the Philippines was investigated in terms of safety and efficacy. METHODS Prior to the vaccination campaign, a house-to-house survey was carried out to collect data on the dog population in the study area, the coastal village of Mindoro. During the vaccination campaign all households were visited again, and all dogs e...

Rabies remains an important worldwide health problem. Newcastle disease virus (NDV) was developed as a vaccine vector in animals by using a reverse genetics approach. Previously, our group generated a recombinant NDV (LaSota strain) expressing the complete rabies virus G protein (RVG), named rL-RVG. In this study, we constructed the variant rL-RVGTM, which expresses a chimeric rabies virus G pr...

Considering the high prevalence of rabies in cattle, we aimed to evaluate the interference of colostral antibodies transferred to calves after birth and the benefit of administering an antirabies vaccination in two-month-old calves compared to vaccinating at 4 and 6 months of age. Calves born from females revaccinated against rabies during the third trimester of pregnancy were studied. Forty-ei...

PURPOSE Myeloid differentiation factor 88 (Myd88), a ubiquitous Toll-like receptor adaptor molecule, has been reported to play important roles in B cell responses to infections and vaccination. The present study evaluated the effects of genetic adjuvanting with Myd88 on the immune responses to a plasmid DNA rabies vaccine. MATERIALS AND METHODS Plasmids encoding rabies glycoprotein alone (pIR...