Monitoring extracellular matrix (ECM) components is one of the key methods used to determine tissue quality in three-dimensional scaffolds for regenerative medicine and clinical purposes. Raman spectroscopy can be used for non-invasive sensing of cellular and ECM biochemistry. We have investigated the use of conventional (confocal and semiconfocal) Raman microspectroscopy and fibre-optic Raman ...

Adoption of Quality by Design (QbD) principles, regulatory support of QbD, process analytical technology (PAT), and continuous manufacturing are major factors effecting new approaches to pharmaceutical manufacturing and bioprocessing. In this review, we highlight new technology developments, data analysis models, and applications of Raman spectroscopy, which have expanded the scope of Raman spe...

Raman spectroscopy is a powerful non-destructive technique for qualitatively and quantitatively characterizing materials. However, noise often obscures interesting Raman peaks due to the inherently weak Raman signal, especially in biological samples. In this study, we develop a method based on spectral reconstruction to recover Raman spectra with low signal-to-noise ratio (SNR). The synthesis o...

We demonstrate the use of spatially offset Raman spectroscopy (SORS) in the identification of counterfeit pharmaceutical tablets and capsules through different types of packaging. The technique offers a substantially higher sensitivity than that available from conventional backscattering Raman spectroscopy. The approach is particularly beneficial in situations where the conventional Raman backs...

Femtosecond Raman spectroscopy has been developed to investigate ultrafast photoinduced structural changes of materials. Vibrational modes in the photogenerated transient species are measured by stimulated Raman scattering using a Raman pump pulse with narrow bandwidth and a femtosecond supercontinuum probe pulse. The Raman signal can be measured without slowing the temporal response and broade...

We demonstrated a continuous-wave (CW) self-Raman laser with high conversion efficiency by using Yb:KGW as the Raman crystal. The first Stokes line of wavelength centered at 1095.2 nm with spectral bandwidth of 8 nm and the cascaded Raman conversion wavelength at 1109.5 nm with spectral bandwidth of 3.4 nm were observed with a Raman shift of 89 cm⁻¹ with respect to the fundamental laser wavelen...

Dielectric stack interference ® lters can be used in conjunction with a high-throughput single-stage spectrograph to facilitate the measurement of high signal-to-noise (S/N) ultraviolet (UV) Raman spectra with 228.9-nm and 244-nm excitation wavelengths. Placed between the sample and the spectrograph, these ® lters re ̄ ect Rayleigh scattering while transmitting Stokes-shifted Raman scattering. W...

An important source of opacity in exoplanet atmospheres at short visible and near-UV wavelengths is Rayleigh scattering of light on molecules. It is accompanied by a related, albeit weaker process – Raman scattering. We analyze the signatures of Raman scattering imprinted in the reflected light and the geometric albedo of exoplanets, which could provide information about atmospheric properties....

Raman spectroscopy and in situ Raman spectroelectrochemistry have been used to study the influence of defects on the Raman spectra of semiconducting individual single-walled carbon nanotubes (SWCNTs). The defects were created intentionally on part of an originally defect-free individual semiconducting nanotube, which allowed us to analyze how defects influence this particular nanotube. The form...

in this research zinc oxide (zno) nano-crystalline powders were prepared by sol-gel method using zinc acetate. the zno nanoparticles were characterized by x-ray diffraction (xrd), scanning electron microscopy (sem), ultraviolet-visible (uv-vis), fourier transform infra-red (ft-ir) and energy dispersive x-ray (edx) spectroscopy. the structure of nanoparticles was studied using xrd pattern. the c...