Peptides selected from phage-displayed random peptide libraries are valuable in two aspects. On one hand, these peptides are candidates for new diagnostics, therapeutics and vaccines. On the other hand, they can be used to predict the networks or sites of protein-protein interactions. MimoDB, a new repository for these peptides, was developed, in which 10,716 peptides collected from 571 publica...

In 1990, a questionnaire was mailed to all physicians in four counties in the lower Rio Grande Valley of Texas and to a random sample of physicians in Bexar County, Texas (San Antonio). Two hundred and eighty of 573 Valley physicians (48.9%) and 162 of 273 Bexar County physicians (59.3%) responded to the survey, for an overall response rate of 52.2%. The two groups were compared primarily to de...

One Page Abstract Oligopeptides are becoming increasingly useful tools in molecular biology. For example, combinatorial random peptides have been used to great effect in drug development, while peptide libraries have been used to investigate active binding motifs. Cellular assays with short oligopeptides have been used to investigate biological signalling pathways. By palmitoylating the N-termi...

Despite an abundance of peptides inside a cell, only a small fraction is ultimately presented by HLA-I on the cell surface. The presented peptides have HLA-I allomorph-specific motifs and are restricted in length. So far, detailed length studies have been limited to few allomorphs. Peptide-HLA-I (pHLA-I) complexes of different allomorphs are qualitatively and quantitatively influenced by tapasi...

Production of cell-targeting vectors in part involves the addition of new targeting ligands to the vector to mediate binding to the cells of interest. For viral vectors, the ideal approach is to genetically engineer new ligands into the capsid proteins of the virus to generate a single agent to mediate therapy. Although this is ideal, this insertion of an exogenous ligand from one structural co...

Phage display is a powerful technique for profiling specificities of peptide binding domains. The method is suited for the identification of high-affinity ligands with inhibitor potential when using highly diverse combinatorial peptide phage libraries. Such experiments further provide consensus motifs for genome-wide scanning of ligands of potential biological relevance. A complementary but con...

Protein-phosphorylation is a post-translation modification that alters the binding or the enzymatic properties of the modified proteins. In particular, phosphorylation of tyrosine residues is a crucial event in protein-protein interactions occurring during intracellular signal transduction. Several approaches have been used to study these interactions. Combinatorial libraries have proven to be ...

Increased multiple antibiotic resistance in the face of declining antibiotic discovery is one of society's most pressing health issues. Antimicrobial peptides represent a promising new class of antibiotics. Here we ask whether it is possible to make small broad spectrum peptides employing minimal assumptions, by capitalizing on accumulating chemical biology information. Using peptide array tech...

A novel method for the parallel synthesis of peptide-biocargo conjugates was developed that utilizes affinity purification for fast isolation of the conjugates in order to avoid time consuming HPLC purification. The methodology was applied to create two libraries of cell-penetrating peptide (CPP)-PNA705 conjugates from parallel-synthesized peptide libraries. The conjugates were tested for their...

The study of protease specificity provides information on active-site structure and function, protein-protein interaction, regulation of intracellular and extracellular pathways, and evolution of protease and substrate genes. Peptide libraries that include fluorogenic and binding tags are often generated by solid-phase synthesis. Even larger explorations of cleavage site preferences employ posi...