Polycomb-group (PcG) proteins mediate repression of developmental regulators in a reversible manner, contributing to their spatiotemporally regulated expression. However, it is poorly understood how PcG-repressed genes are activated by developmental cues. Here, we used the mouse Meis2 gene as a model to identify a role of a tissue-specific enhancer in removing PcG from the promoter. Meis2 repre...

The serum amyloid A (SAA) superfamily comprises a number of differentially expressed genes with a high degree of homology in mammalian species. SAA4, an apolipoprotein constitutively expressed only in humans and mice, is associated almost entirely with lipoproteins of the high-density range. The presence of SAA4 mRNA and protein in macrophage-derived foam cells of coronary and carotid arteries ...

Adeno-associated virus (AAV) integrates site specifically into the AAVS1 locus on human chromosome 19. Although recruitment of the AAV nonstructural protein Rep78/68 to the Rep binding site (RBS) on AAVS1 is thought to be an essential step, the mechanism of the site-specific integration, particularly, how the site of integration is determined, remains largely unknown. Here we describe the ident...

Many bacterial type I toxin mRNAs possess a long 5΄ untranslated region (UTR) that serves as the target site of the corresponding antitoxin sRNA. This is the case for the zorO-orzO type I system where the OrzO antitoxin base pairs to the 174-nucleotide zorO 5΄ UTR. Here, we demonstrate that the full-length 5΄ UTR of the zorO type I toxin hinders its own translation independent of the sRNA where...

This study presents efficient algorithms for performing multiply-by-3 (3N) and divide-by-3 (N/3) operations with the additions and subtractions, respectively. No multiplications and divisions are needed. Full adder (FA) and full subtractor (FS) can be implemented to realize the N3 and N/3 operations, respectively. For fast hardware implementation, this paper introduces two basic cells UCA and U...

Background: There was a need for a simple method of predicting the severity of gallstone pancreatitis soon after admission, to guide the clinician for intensive monitoring, or for transfer to a specialist center. Aim: To validate the role of simplified criteria [white blood cell count (WBC) > 14.5 x 10/dl; blood urea nitrogen (BUN) > 12 mg/dl; random blood sugar (RBS) > 150 mg/dl; pulse > 100/m...

Robert syndrome (RBS) and Cornelia de Lange syndrome (CdLS) are human developmental disorders characterized by craniofacial deformities, limb malformation and mental retardation. These birth defects are collectively termed cohesinopathies as both arise from mutations in cohesion genes. CdLS arises due to autosomal dominant mutations or haploinsufficiencies in cohesin subunits (SMC1A, SMC3 and R...

Standard numerical simulations for optimization or inverse identification of welding processes remain costly and difficult due to their multi-parametric aspect and inherent complexity. The aim of this paper is to propose a non-intrusive strategy for building computational vademecums dedicated to real-time simulations of nonlinear thermo-mechanical problems. There is in essence, a set of precomp...

*State Key Lab. of Automotive Energy and Safety Tsinghua University, Beijing, China 100084 Phone: 010-62771839 Regenerative Braking Systems (RBS) provide an efficient method to assist hybrid electric buses achieve better fuel economy while lowering exhaust emissions. This paper describes the design and testing of three regenerative braking systems, one of which is a series regenerative braking ...

The aim of the present study was to investigate the factor structure and psychometric properties of the Polish adaptation of Romantic Beliefs Scale (RBS; Sprecher & Metts, 1989). In a sample of 414 Polish university students aged 19-25 (227 females and 187 males), the factor structure of the original English version was confirmed for the four subscales: Love Finds a Way, One and Only, Idealizat...