AIM The aim of the study was in vitro analysis of biological activity of recombinant human beta-defensin-4 (rec-hBD-4). METHODS hBD-4 cDNA was cloned into pGEX-2T vector, and recombinant plasmid was transformed into E. coli BL21(DE3) cells. To purify soluble fusion GST-hBD-4 protein, affinity chromatography was applied. Rec-hBD-4 was cleaved from the fusion protein with thrombin, and purified...

T H E histidine-3 (his-3) locus of Neurospora crassa has aroused considerable interest because it 'controls not only the last step but also at least two early reactions in the biosynthesis of histidine (AHMED, CASE and GILES 1964; CATCHESIDE 1960, 1965). Complementation and genetic analyses of his-3 mutants, showing that noncomplemeiiting mutants, lacking all the three functions, appeared to be...

According to ideomotor theory, action planning is based on anticipatory perceptual representations of action-effects. This aspect of action control has been investigated in studies using the response-effect compatibility (REC) paradigm, in which responses have been shown to be facilitated if ensuing perceptual effects share codes with the response based on dimensional overlap (i.e., REC). Addit...

BACKGROUND It has been suggested that colorectal cancer be regarded as several subgroups defined according to tumor location rather than as a single entity. The current study aimed to identify the most useful method for grouping colorectal cancer by tumor location according to both baseline and survival characteristics. METHODS Cases of pathologically confirmed colorectal adenocarcinoma diagn...

The aim of this study was to determine the effect of increasing the duration of the recovery periods separating serial bouts of small sided games (SSG) of football on technical skills (TS). Twelve semi-professional footballers (mean ̆ SD; age 21 ̆ 3 years; VO2peak 64 ̆ 7 mL ̈min ̈kg ́1; playing experience 15 ̆ 3 years) completed two SSG sessions, consisting of 3 vs. 3 players and 6 bouts of 2 min,...

This paper describes the establishment of and the issues experienced by the Research Ethics Committee (REC) of a Business School within a University in Ireland. It identifies the issue of voluntarily given informed consent as a key challenge for RECs operating in a Business School context. The paper argues that whilst the typology of ethical issues in business research are similar to the wider ...

Two experiments examined the effects of a saccharin-based artificial sweetener (Sucram) on health, performance, and dietary preference of feedlot cattle. In Exp. 1, 200 steer calves (initial BW = 190.4 +/- 1.47 kg) were fed a 65% concentrate diet supplemented with or without 200 mg of Sucram/kg (DM basis) during a 56-d receiving-growing period. Feeding Sucram did not affect overall (P = 0.19) D...

OBJECTIVE Disruption of endothelial barrier integrity is a characteristic of many inflammatory conditions. However, the origin and function of endothelial cells (ECs) restoring endothelial barrier function remain unknown. This study defined the roles of resident ECs (RECs) and bone marrow-derived endothelial progenitor cells (BMDEPCs) in endothelial barrier restoration after endotoxemic lung in...

Renewable Energy Consumption (REC) would reduce pollution and a large pool of literature has probed the Environmental Kuznets Curve (EKC) including REC in panel or country-specific model. The present study reviewed 69 empirical studies found that 57 out validated EKC but 12 did not confirm EKC. Out these, 64 reduced emissions. In analyses, 18 25 24 substantiated studies, 39 44 40 Comparatively,...