Real-time polymerase chain reaction (PCR) is one of the most sensitive and accurate methods for quantifying transcript levels especially for those expressed at low abundance. The selective amplification of target DNA over multiple cycles allows its initial concentration to be determined. The amplification rate is a complex interplay of the operating conditions, initial reactant concentrations, ...

The ethiological factor of periodontal diseases is the presence periodontopathogens; in state imbalance with commensals they begin to affect pathologically. With a decrease number periodontopathogens biofilm, it possible restore balance and prevent or their transition stage remission. Nowadays, most informative accessible diagnostic method for determining real-time polymerase chain reaction (PC...

Real-time fluorescence polymerase chain reaction (PCR) techniques are increasingly used to quantitate target sequences for diagnostic and research purposes. Currently, the so called TaqMan probe chemistry is mostly used as fluorogenic system. This probe format is strictly dependent on the 5'-exonuclease activity of DNA polymerase as fragmentation of the probe during the reaction is essential fo...