hepatitis c virus (hcv) is an important agent causing chronic liver infection, which often leads to liver cirrhosis and lethal hepatocellular carcinoma (hcc). at present, there is no effective hcv vaccine for prevention of hepatic disease and the standard treatment is neither economical nor fully effective in all the patients. however, vaccination based on structural and nonstructural proteins ...

We examined the biotransformation of enantiomeric pairs of enones such as pulegone and carvone in recombinant Escherichia coli expressing Nicotiana tabacum pulegone reductase. It was found that recombinant E. coli cells acquired the ability for stereospecific hydrogenation of the exocyclic C=C double bond of pulegone. However, stereospecificity in hydrogenation with the recombinant E. coli cell...

in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...

background contamination of therapeutic recombinant proteins with residual host cell dna must be controlled under the regulatory standards. objectives the current study established a new rapid, sensitive real time polymerase chain reaction (pcr) approach to measure the reliably of the residual escherichia coli (e. coli) host cell genomic dna in the recombinant streptokinase and alfa interferon ...

Background The use of Escherichia coli for the production of recombinant proteins is an established strategy to obtain biotechnological tools. However, the recombinant protein expression is dependent on temperature, bacterial culture time, induction period, nutrients, plasmid characteristics, and insert itself. Thus, the aim of this study was optimize the expression of recombinant gE protein (r...

The recombinant proteins are under special attention due to their medical, pharmaceutical and industrial uses. Bacteria are simple and cost effective hosts for producing recombinant proteins. The Gram-negative bacterium Escherichia coli is a high yielding recombinant protein production system, due to its specific characteristics such as the rapid and high-density growth, need of poor medium and...