Endoglucanase 2 (EG2) of the cellulolytic ruminal anaerobe Bacteroides succinogenes is a 118-kilodalton (kDa) enzyme which binds to cellulose and produces cellotetraose as the end product of hydrolysis. The purified enzyme was treated with the protease trypsin in an attempt to isolate peptides which retained the ability to either hydrolyze soluble carboxymethyl cellulose or bind to insoluble ce...

A hippurate-negative biovariant of Brachyspira pilosicoli (B. pilosicolihipp-) is occasionally isolated in diarrhoeic pigs in Finland, often concomitantly with hippurate-positive B. pilosicoli or Lawsonia intracellularis. We studied pathogenicity of B. pilosicolihipp- with special attention paid to avoiding co-infection with other enteric pathogens. Pigs were weaned and moved to barrier facilit...

anxiety is an emotion characterized by an unpleasant manner of inner distress, often accompanied by nervous behavior. anxiety is related to the particular behaviors of fight or flight responses. the amygdala, the insula and anterior cingulate cortex seem to be critical, and all three have been referred to as the fear network .the amygdala which plays a fundamental role in neural network of fear...

Twelve intestinal spirochete strains of porcine origin were characterized on the basis of their phenotypic properties, by multilocus enzyme electrophoresis, and by pathogenicity testing in gnotobiotic pigs. The spirochetes used included two strains of Serpulina hyodysenteriae (B204 and P18A), two strains of Serpulina innocens (B256 and 4/71), one strain from the proposed new genus and species "...

Exclusive cross sections for the $^{43}\mathrm{P}(\ensuremath{-}1p)^{42}\mathrm{Si}$ reaction to lowest ${0}^{+}$ and ${2}^{+}$ states, measured at NSCL with GRETINA S800, are interpreted in terms of a two-level mixing (collective) model oblate prolate coexisting shapes. Using formalism developed deformed nuclei, we calculate spectroscopic amplitudes exclusive strong-coupling limit, where $^{43...

BACKGROUND B. subtilis is an important organism in the biotechnological application. The efficient expression system is desirable in production of recombinant gene products in B. subtilis. Recently, we developed a new inducible expression system in B. subtilis, which directed by B. subtilis maltose utilization operon promoter Pglv. The system demonstrated high-level expression for target protei...