objective: acinetobacter baumannii (a. baumannii) has a good potential to colonize on various surfaces. as a virulence factor, adhesion to surfaces is the first step in colonization. the two-partner secretion system (tps) proteins are key factors for bacterial attachment. the purpose of this study is to identify and study the role of this family of proteins in adhesion of a. baumannii to human ...

nowadays, production of recombinant proteins in eukaryotes is gaining good deal of attention. transgenic chicken as a eukaryotic system has a high potential for producing recombinant proteins. post-translational changes, especially glycosylation, are characteristic of the eukaryotic proteins. in practice we need to choose a proper expressing host when considering over-expression of a recombinan...

infectious bursal disease virus (ibdv) is the causative agent of gumboro disease, an infectious disease of global economic importance in poultry. the expression of heterologous proteins in p.pastoris is fast, simple and inexpensive. in this study, vp2 encoding gene of classical d78 ibdv was amplified using reverse transcription (rt) polymerase chain reaction (pcr) and cloned into ppiczαa vector...

Background: Plants have various defense mechanisms such as production of antimicrobial peptides, particularly pathogenesis related proteins (PR proteins). PR10 family is an essential member of this group, with antifungal, antibacterial and antiviral activities.Objective: The goal of this study is to assess the antifungal activity of maize PR10 against some of fungal phytopathogens.M...

introduction: at present, most recombinant proteins are produced in prokaryotes especially e coli. yeasts and cho also are used as eukaryotic hosts. leishmania tarentolae, a parasite of lizards, a member of trypanosomatidae family is one of the new systems for expression of heterologous proteins. in this system, some of the parasitic protozoa features are used in expression of mammalian protein...

Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...

Diversity among the pathogenic strains of Theileria equi (T. equi), a major agent of equine piroplasmosis, can affect the appropriate detection of parasite and host immunization. Production of recombinant surface proteins from an infected horse in natural endemic area provides a reliable tool for immunodiagnosis of parasite. Regarding this, the present study was targeted toward the cloning, exp...