We established polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and diagnostic PCR based on cytochrome C oxidase subunit I (COI) barcodes of Bungarus multicinctus, genuine Jinqian Baihua She (JBS), and adulterant snake species. The PCR-RFLP system utilizes the specific restriction sites of SpeI and BstEII in the COI sequence of B. multicinctus to allow its cleavage ...

Toxoplasmosis, caused by Toxoplasma gondii, is a parasitic zoonosis with worldwide distribution. The present study investigated the prevalence of T. gondii in dogs in Zhanjiang city, southern China, using both serological and molecular detection. A total of 364 serum samples and 432 liver tissue samples were collected from the slaughter house between December 2012 and January 2013 and were exam...

background hepatitis delta virus (hdv) is dependent on the hepatitis b virus for transmission and propagation. based on isolated hdv sequences from different parts of the world, at least three major different genotypes with different geographic distributions are suggested. studies have shown that genotype 1 is the predominant genotype of hdv in different parts of iran; however, the genotype dis...

conclusions it seems that pcr-rflp is a suitable technique for the identification of mucorales at the species level. results on the basis of the molecular and morphological data, we identified mucor plumbeus (10.83%), m. circinelloides (9.17%), lichtheimia corymbifera (9.17%), m. racemosus (5.83%), m. ramosissimus (3.33%), and l. blakesleeana (0.83%). objectives the present study was conducted ...

ackground: Knowing the human platelet antigens (HPA) and genes frequency in different populations is important not only for population studies but also for clinical transfusion practice. HPA genes frequency in Iran is not evaluated, so we investigated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 patie...

conclusions the results of antifungal susceptibility tests demonstrated that voriconazole was the most active drug against candida isolates. candida albicans is the most common yeast isolated from human blood world-wide; the unexpectedly higher rate of c. parapsilosis isolated in this study necessitates more studies with larger sample sizes in iran. results candida parapsilosis (c. parapsilosis...

background: sulfatase 1 ( sulf1 ) function is to remove the 6-o-sulphate group from heparan sulfate. this action changes the binding sites of extracellular growth factors. sulf1 expression has been reported to be changed in angiogenesis. we hypothesized that single nucleotide polymorphisms (snps) of sulf1 would impact clinicopathologic characteristics. objective: study of sulf1 gene polymorphis...

Soil fungal community structures are often highly heterogeneous even among samples taken from small field plots. Sample pooling is widely used in order to overcome this heterogeneity, however, no objective criteria have yet been defined on how to determine the number of samples to be pooled for representatively profiling a field plot. In the present study PCR/RFLP and T-RFLP analysis of fungal ...

Garlic belongs to the Allium genus, which includes more than 750 species divided into 60 taxonomic groups. It is cultivated in many countries throughout world for bulb and used as a spice functional food. The plant vegetatively propagates. This review will focus on origins, biology, analysis of genetic diversity, pharmacological properties garlic. appears from this synthesis that sativum derive...