Dengue virus was detected for the first time in Chile, in an outbreak of dengue fever on Easter Island. The virus was isolated in tissue culture and characterized by reverse transcription-polymerase chain reaction as being dengue type 1.

Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...

the biological mechanisms of tooth movement are based on the response of periodontal tissues to mechanical forces. the final result of these responses is remodeling of the extracellular matrix. tissue reactions may vary depending upon the type, magnitude and duration of the applied forces. the purpose of the present study was to analyze the effects of centrifugal force on the transcription of c...

background human enteroviruses are members of picornaviridae family; they are non-enveloped, icosahedral viruses with positive rna as genome. echovirus 30 is an important member of enteroviruses that is recognized in outbreaks of enterovirus meningitis. objectives the aim of this study was to determine relative frequency of echovirus 30 as an important agent of aseptic meningitis among children...

Abortion in dairy cattle is generally defined as the loss of fetus on days 47-265 of pregnancy and can significantly reduce the income and capital gain of farmers and producers. Therefore, sufficient economical support and preventive measurements are essential for this issue. In this study, genetic materials were extracted from the abomasum rennet, as well as homogenous preparations of brain, k...

The extraction of intact RNA from oocyte is quite challenging and time-consuming. A standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in oocytes. In the past, several attempts in getting RNA for gene expression study ended up with a few different modified methods. Extraction of high-quality RNA from oocyte is important before further downstream analyses such as...

A novel GII.17 norovirus emerged in Asia in the winter of 2014/15. A worldwide spread is conceivable and norovirus diagnostic assays need to be evaluated to investigate if they adequately detect this emerging genotype. Seven immunochromatographic kits commercially available in Europe were evaluated on ten stool samples where GII.17 virus had been quantified by real-time reverse transcription-po...

We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing unbound MB. This module consists microfluidic channel with shape funnel operated electrohydrodynamic actuation. report limit detection 2 pM in less than 1 min miR-21 alone, then demonstrate that levels, measured fine needle biopsy samples, fr...

Beta catenin gene can inhibit the chondrogenic differentiation of human adipose tissue derived stem cells, while sex determining region Y-box9 promote cells. However, it is still necessary to study role combination two genes in early The purpose this was investigate whether knockdown and overexpression combined with modification cells could Therefore, divided into four groups or lentivirus vect...

BACKGROUND The reverse transcription (RT) of RNA to cDNA is a necessary first step for numerous research and molecular diagnostic applications. Although RT efficiency is known to be variable, little attention has been paid to the practical implications of that variability. METHODS We investigated the reproducibility of the RT step with commercial reverse transcriptases and RNA samples of vari...