Giardia duodenalis is one of the most prevalent enteroparasites in children. This parasite produces several clinical manifestations. The aim of this study was to determine the prevalence of genotypes of G. duodenalis causing infection in a region of southeastern Mexico. G. duodenalis cysts were isolated (33/429) from stool samples of children and molecular genotyping was performed by polymerase...

Actinobacillus pleuropneumoniae is a causative agent of porcine pleuropneumonia that is one of the important bacterial diseases of the respiratory tract of pigs. The disease occurs worldwide and results in serious economic losses to the pig-rearing industry. In this review, new typing methods based on the antigenic and genetic diversity of a protective outer membrane lipoprotein (OmlA), that ha...

Molecular phylogeny and genetic diversity in all the nine species (Rhizophora mucronata, R. apiculata, R. stylosa, Bruguira cylindrical, B. parviflora, B. gymnorriza, Ceriops tagal and C. decandra) and a natural Rhizophora hybrid, of the mangrove Rhizophoraceae, occurring in the Indian sub-continent were analysed using random amplified polymorphic DNA (RAPD), restriction fragment length polymor...

Genetic variation within and among wild Turkey, Meleagris gallopavo, subspecies was measured using DNA sequencing and PCR-RFLP analysis of a 655 bp portion ofthe mtDNA D-Ioop region. DNA sequencing from 25 individual Eastern, Merriam, Rio Grande and domestic Turkeys revealed 16 polymorphic sites. Parsimony and neighbor-joining analysis did not show support for the four wild Turkey subspecies st...

BACKGROUND Due to the differences in the oncogenic activity of human papillomaviruses (HPV), it is clinically important to accurately identify HPV types in a simple and time effective manner. OBJECTIVES We aimed at developing a straightforward and cost-effective assay to individually identify all mucosal HPVs, based on the amplification of L1 gene using MY09/11 primers, and subsequent restric...

AIM To develop polymerase chain reaction based artificial restriction fragment length polymorphism (artificial RFLP PCR) assays for DR3 and DR4 alleles of the multiallelic DRB1 locus and to apply them to paraffin wax embedded archival material. METHODS Sixty five samples from DRB1 typed cell lines were analysed using the artificial RFLP PCR method to determine the specificity and sensitivity ...