The gene chiA, which codes for endochitinase, was cloned from a soilborne Enterobacter agglomerans. Its complete sequence was determined, and the deduced amino acid sequence of the enzyme designated Chia_Entag yielded an open reading frame coding for 562 amino acids of a 61-kDa precursor protein with a putative leader peptide at its N terminus. The nucleotide and polypeptide sequences of Chia_E...

As stated in the above-mentioned paper (pi), several writers have reported a root rot or wilt of alfalfa with which a species of Fusarium has been associated. Cottam (3) states that the pathogenicity of a root disease of alfalfa in Utah has been traced to the activity of a Fusarium. He describes this disease as being characterized by brown cankerous depressions at the bases of lateral roots, wh...

A new strain, SD12, was isolated from tannery waste polluted soil and identified as Pseudomonas aeruginosa on the basis of phenotypic traits and by comparison of 16S rRNA sequences. This bacterium exhibited broad-spectrum antagonistic activity against phytopathogenic fungi. The strain produced phosphatases, cellulases, proteases, pectinases, and HCN and also retained its ability to produce hydr...

Estimation and prediction in generalized linear mixed models are often hampered by intractable high dimensional integrals. This paper provides a framework to solve this intractability, using asymptotic expansions when the number of random effects is large. To that end, we first derive a modified Laplace approximation when the number of random effects is increasing at a lower rate than the sampl...

The soil-borne ascomycete fungus Cylindrocarpon destructans causes ginseng root rot disease and produces various secondary metabolites such as brefeldin A and radicicol. The slow growth of this fungus compared with other plant pathogenic and saprophytic fungi in soil disturbs isolation of this fungus from soil and infected ginseng. In this study, we developed a selective medium for C. destructa...

A Gram-negative bacterial isolate, named PG4, was recovered from the rhizosphere of a tobacco plant (Nicotiana tabacum cv. Burley) and was identified as Lysobacter capsici based on the sequencing and phylogenetic analysis of 16S rRNA and characteristics in the Biolog Identification System. Strain PG4 showed chitinase and protease activity and was able to inhibit the growth of a broad range of p...