The arginine-rich domains of several RNA-binding proteins have been shown to bind their cognate RNAs with high affinities and specificities as isolated peptides, adopting different conformations within different complexes. The sequence simplicity and structural diversity of the arginine-rich motif has made it a good framework for constructing combinatorial libraries and identifying novel RNA-bi...

HIV-1 Rev is an ~13 kD accessory protein expressed during the early stage of virus replication. After translation, Rev enters the nucleus and binds the Rev response element (RRE), a ~350 nucleotide, highly structured element embedded in the env gene in unspliced and singly spliced viral RNA transcripts. Rev-RNA assemblies subsequently recruit Crm1 and other cellular proteins to form larger comp...

Decoy receptor 3 (DcR3) is a soluble decoy receptor belonging to the tumor necrosis factor receptor superfamily that is overexpressed in various malignant tumor types. DcR3 has been implicated in tumor cell survival by inhibiting apoptosis and by interfering with immune surveillance. A previous study showed that DcR3 expression is associated with Epstein-Barr virus (EBV)-positive lymphomas but ...

Chestnut rose, R. roxburghii Tratt. (Rosaceae) (RR) is an important crop in China due to its nutritional and medicinal values. RR frequently produces trichomes on the surfaces of a diverse range organs, however genetic component exists control trichome development, with some cultivars having significantly fewer others. Certain varieties have fruits that are thickly covered macroscopic trichomes...

Abstract. Modeling water flow in unsaturated soils is vital for describing various hydrological and ecological phenomena. Soil dynamics described by well-established physical laws (Richardson–Richards equation – RRE). Solving the RRE difficult due to inherent nonlinearity of processes, numerical methods have been proposed solve issue. However, applying practical situations very challenging beca...

Human immunodeficiency virus (HIV) and all other lentiviruses utilize the essential viral protein Rev, which binds to RRE RNA, to export their unspliced and partially spliced mRNAs from the nucleus. We used a rev- and RRE-defective HIV type 1 (HIV-1) molecular clone in complementation experiments to establish a method for the rapid isolation of posttranscriptional regulatory elements from the m...

BACKGROUND AND PURPOSE Early recurrent strokes lead to extended hospitalization and high number of complications. We investigated three stroke scores, the Essen Stroke Risk Score (ESRS), the ABCD(2) and the Recurrence Risk Estimator at 90 days (RRE-90) for their prognostic value to predict early recurrent stroke, death and progressive stroke. METHODS Clinical and radiological data from 1727 c...

The Rev protein of human immunodeficiency virus type 1 (HIV-1) binds to an RNA structure, the Rev-responsive element (RRE), to enhance expression of the viral structural genes by relieving the nuclear sequestration of incompletely spliced viral transcripts. It has been suggested that nuclear retention of these mRNAs, in mammalian cells, is due to the activity of either cis-acting repressive seq...

Ad Addr dr res es ess s s fo fo for r Co Co orr rr rre es espo po pond nd nde ence ce e: : : Marzia Leacc cc cche he he M M MD D D

Current estimators of variance for the k nearest neighbours (kNN) technique are designed for estimates of population totals. Their efficiency in small-area estimation problems can be poor. In this study, we propose a modified balanced repeated replication estimator of variance (BRR) of a kNN total that performs well in small-area estimation problems and under both simple random and cluster samp...