background: immunochromatographic based rk39 antibody detection test became popular for the diagnosis of vis­ceral leishmaiasis (vl) because of high sensitivity, rapidity, easy to interpret, and cost effectiveness. however, false positive result after complete cure of the patients is the major limitation with this test. the aim of the study to access the usefulness of non-invasive samples i.e. ...

A pilot study was carried out on the detection of trypanosome-specific antibodies in saliva for diagnosis of sleeping sickness. All twenty-three saliva samples of parasitologically confirmed Trypanosoma brucei gambiense patients tested positive in an indirect enzyme-linked immunosorbent assay, whereas all 14 saliva samples of a negative control group remained negative. Trypanosome-specific anti...

Saliva and polymorphonuclear leukocytes (PMNs) are both important players in the innate defense of the oral cavity, but little is known of the effect of saliva on PMN function. In this study, we investigated the effect of saliva on the adhesion of Fu-sobacterium nucleatum and Candida albicans to human granulocytic-differentiated HL-60 cells. The adhesion of FITC-labeled F. nucleatum and C. albi...

Trypanosoma cruzi, the etiological agent of Chagas disease, is transmitted by bug feces deposited on human skin during a blood meal. However, parasite infection occurs through the wound produced by insect mouthparts. Saliva of the Triatominae bug Rhodnius prolixus is a source of lysophosphatidylcholine (LPC). Here, we tested the role of both triatomine saliva and LPC on parasite transmission. W...

We examined the frequency of HCV-RNA in saliva samples from anti-HCV positive patients. Both plasma and saliva samples from 39 HCV patients (13 with normal liver enzymes, 19 with abnormal liver enzymes and 13 with cirrhosis) were investigated. Stimulated saliva and fresh plasma were centrifuged (900 x g,10 min) and stored at -70 degrees C, after the addition of guanidine isothiocyanate RNA extr...

This study aimed to clarify the ability of the buffer systems of saliva to inhibit enamel demineralization after intake of an acid beverage. In the first experiment, titrable acidity tests were carried out. Ten milliliters of saliva stimulated by chewing gum base was obtained from 10 healthy adult subjects and the pH of each saliva sample was measured. The beverages used for the experiment were...

Background: Trace elements like iron, zinc, magnesium, cobalt and manganese are some of these elements involved in antioxidant defense mechanism. Many authors observed direct association between trace elements deficiency and the cancer mortality. The study was conducted to measure levels of Zinc (Zn), Copper (Cu), Iron (Fe), Manganese (Mn), Cobalt (Co), Nickel (Ni), Chromium (Cr), Cadmium (Cd),...

BACKGROUND In resource limited settings access to laboratory monitoring of HIV treatment is limited and therapeutic drug monitoring is generally unavailable. This study aimed to evaluate nevirapine concentrations in saliva using low-cost thin-layer chromatography (TLC) and nevirapine concentrations in plasma and saliva using high performance liquid chromatography (HPLC) methods; and to correlat...

PURPOSE The aim of the present study was to evaluate the alterations of surface microhardness and wear caused by the sodium bicarbonate jet on bovine enamel and the further remineralizing effect of artificial saliva. METHODS Fifteen enamel samples (4,0mm x 4,0mm) were used, which constituted the groups: no treatment (MI); treatment with sodium bicarbonate jet (MII and DI); treatment with sodi...

Introduction: Human beings produce saliva, which is a vital secreted fluid. A significant quantity of saliva left on the skin while biting, sucking, or licking. This if effectively detected could be utilized as forensic evidence. DNA extraction and typing complex expensive technique; hence, ultraviolet (UV) spectroscopy used an effective tool in detecting saliva. Aim: The aim was to detect pres...