The ten principles of GSP are presented with the aim establishing a road map toward development overall greener analytical methodologies. Paramount aspects for greening sample preparation and their interconnections identified discussed. These include use safe solvents/reagents materials that renewable, recycled reusable, minimizing waste generation energy demand, enabling high throughput, minia...

Lipid standards were purchased from Avanti Polar Lipids, Inc., and Matreya LLC and a list is included in the supplementary information section. A final concentration of 1 µM was desired for the LC-IMS-MS studies. The standards were diluted with chloroform to reach the desired concentration. River Laboratory (Raleigh, NC). Females were mated with fertile males of the same strain to induce pregna...

In eukaryotic cells, protein synthesis occurs at the rate of 6–9 amino acid residues per second. With a median length of 360 amino acids, the synthesis of an " average " protein takes about a minute to complete [1]. At this rate, the synthesis of a single molecule of the muscle protein titin, being over 34,000 residues in length, requires over two hours to complete [2]. While this seems slow as...

Joachim Mayer, Lucille A. Giannuzzi, Takeo Kamino, and Joseph Michael preparation can be applied to almost any material type—hard, soft, or combinations thereof. The number of materials for which successful TEM sample preparation with FIBs has been documented certainly reaches several hundred and spans from hard matter such as metals, ceramics, and composites to soft matter including polymers, ...