An investigation into the methanol extracts obtained from stems of Dodonaea viscosa led to isolation one nor-clerodane diterpene (1) and two labdane diterpenes (2, 3), as well 17 known compounds (4–20). The structures these were elucidated based on chemical spectral evidence. stereochemical structure was confirmed via its circular dichroism spectrum calculated electronic spectrum. Isolated eval...

The chromatographic separation of a methylene chloride extract of Artemisia rubripes led to the isolation of a new sesquiterpene lactone (3), together with four known compounds, a coumarin (2) and three terpenes (1, 4, and 5). Their structures were characterized to be 1beta,6alpha-dihydroxy-4(15)-eudesmene (1), scopoletin (2), 1alpha,4beta-dihydroxy-8alpha-acetoxy-guaia-2,10(14), 11(13)-triene-...

The relative increase of the 7-ethoxycoumarin-O-deethylase and the 6,7-dimethoxycoumarin-O-demethylase activities were found 93 and 236% using a reconstituted cytochrome P4502B1:NADPH-P450 reductase system by adding to the reaction mixtures their own products. The assays were irradiated during the reactions with the excitation wavelength maximum of their products umbelliferone (lambda E = 365 n...

Cuscuta campestris is a parasitic weed species with noxious effects in broadleaf crops worldwide. The control of the majority affected limited or non-existing. We tested, for first time, effect eighteen metabolites vitro-grown seedlings. found that 2-benzoxazolinone, hydrocinnamic acid and pisatin caused strongest inhibition seedling growth. In addition to growth, necrosis seedling, occurring m...

Continuing our interest in the Uncaria genus, the phytochemistry and the in-vitro α-glucosidase inhibitory activities of Malaysian Uncaria cordata var. ferruginea were investigated. The phytochemical study of this plant, which employed various chromatographic techniques including recycling preparative HPLC, led to the isolation of ten compounds with diverse structures comprising three phenolic ...

The initial glucuronidation rates were determined for eight recombinant human UDP-glucuronosyltransferases (UGTs) of the 1A subfamily, and the bisubstrate kinetics and inhibition patterns were analyzed. At low substrate concentrations, the reactions followed general ternary complex kinetics, whereas at higher concentrations of both substrates, the reactions were mostly characterized by ternary ...

A blue-LED-driven two-liquid-phase system has been set up for the in situ activation of horseradish peroxidase avoiding use hydrogen peroxide and drawbacks related to enzyme denaturation undesired radical side-reactions. The photobiocatalytic was applied oxidative coupling natural synthetic coumarins bicoumarins, allowing obtain homodimers only one step, tedious protecting groups. Two C-2 symme...