Pyruvate oxidase is a peripheral membrane enzyme which has been purified to homogeneity from Escherichia coli. When assayed in the presence of some amphiphiles the enzymatic activity is stimulated nearly 25fold; these amphiphiles include phospholipids, neutral detergents, and charged detergents. Both anionic and cationic detergents, at concentrations well below their critical micelle concentrat...

The linker histones (H1 or H5) which play a key role in the folding of chromatin, are general repressors of gene expression. Nuclei of the mature chicken erythrocytes (and in some mammalian cells) contain both of them. Although the interaction of H5 with DNA is stronger than that of H1, it does not prevent the transcription of some erythroid-specific genes. It has been shown that some modificat...

The aim of the present study was to evaluate anxiety and depression status lung cancer patients during coronavirus disease 2019 (COVID‑19) pandemic effects psychological counseling online. For this purpose, following enrollment. were divided into an intervention group (group I) a control C). Participants in two groups scored using Self‑Rating Anxiety Scale (SAS) Depression (SDS) before one week...

Software testing is the methodology of analyzing nature software to test if it works as anticipated so boost its reliability and quality. These two characteristics are very critical in applications present times. When testers want perform scenario evaluations, oracles generally employed third phase. Upon case execution outcome generation, essential validate results establish behavior’s correctn...

Monoclonal antibody TG1 recognizes specifically antigens HLA-B27, B7, B22 and B17 on cell surface in cytotoxicity and cytofluorometry tests. When cell detergent extracts were subjected to SDS PAGE under mild conditions (no heating and no reduction of the sample) followed by Western blotting, TG1 detected exclusively a complex of B27 heavy chains with beta(2)-microglobulin (as a 50 kDa band) whe...

This paper describes the recent effort to improve our Domain Knowledge Manager (DKM) that is part of a mixed-initiative task based Spoken Dialogue System (SDS) architecture, namely to interact within an ambient intelligence scenario. Machine-learning applied to SDS dialogue management strategy design is a growing research area. Training of such strategies can be done using human users or using ...

A mutant prothrombin, designated prothrombin Tokushima, was purified from plasma of a proband with 12% of normal plasma clotting activity and 42% of normal prothrombin antigen. The purified preparation gave a single band with the same mobility as that of "prothrombin" by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The factor Xa-catalyzed proteolysis of prothrombin Toku...

Prion diseases, known as Transmissible Spongiform Encephalopathies (TSEs), are a group of fatal neuronal, and to some extent infectious disorders, associated with a pathogenic protein agent called prion protein (PrP). The human prion protein (hPrP) fragment encompassing the 91-127 region, also known as the amyloidogenic domain, comprises two copper-binding sites corresponding to His-96 and His-...

Under reducing conditions of SDS-polyacrylamide gel electrophoresis, the CB(1) receptor exists in its monomeric form as well as in an SDS-resistant high molecular weight form that appears to be devoid of G proteins. The CB(1) cannabinoid receptor was immunoprecipitated from 3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate-solubilized rat brain membranes using an antibody against the CB(1)...