EXAMINATION OF NONSTERILE Preparation of Test Strains PRODUCTS: MICROBIAL Use standardized stable suspensions of test strains or prepare as stated below. Seed-lot culture maintenance techENUMERATION TESTS niques (seed-lot systems) are used so that the viable microorganisms used for inoculation are not more than five passages removed from the original master seed-lot. Grow each of the bacterial ...

The growth stage of soybean (Glycine max (L.) Merrill) at the time of inoculation was determined to be a factor in the severity of seed coat mottling caused by Bean pod mottle virus (BPMV, Genus Comovirus, Family Comoviridae). The virus caused seed coat mottling, and reduced seed size and seed weight. The effects of different isolates of BPMV on seed coat mottling in soybean were found to vary ...

Endophytic fungi, which live within host plant tissues without causing any visible symptom of infection, are important mutualists that mediate plant-herbivore interactions. Thrips tabaci (Lindeman) is one of the key pests of onion, Allium cepa L., an economically important agricultural crop cultivated worldwide. However, information on endophyte colonization of onions, and their impacts on the ...

Double inoculation of soybean with Bean pod mottle virus (BPMV), a member of the Genus Comovirus, Family Comoviridae, isolates V-S98-1 and V-S98-15, together with Soybean mosaic virus (SMV) a member of the Genus Potyvirus, Family Potyviridae) isolates 2K-38, S98-51 and S98-52, was compared to single inoculation with each of the isolates, on Hutcheson, Hutcheson Roundup Ready® (RR BC3) and Hutch...

In the present study, the effect of different plant growth promoting rhizobacteria (PGPR) like Azospirillum brasilense and Pseudomonas fluorescens on growth parameters and the production of terpenoid indole alkaloids are investigated in two varieties ‘rosea’ and ‘alba’ of Catharanthus roseus. The production of IAA by the PGPR has taken as main criteria in the selection of PGPRs for the seed and...

introduction considering the nutrient loss, environmental pollution and increase in production costs on account of chemical fertilizer application, supplying mineral nutrients based on organic sources and mycorrhizal inoculation can improve organic matter content in soil (khandan & astaraei, 2005; halajnia et al., 2007), promote sustainable production of oil crops (mostafavian et al., 2008; kaw...

According to Statistics Canada, the cropping area for field pea (Pisum sativa) in Western Canada has increased from 25 000 ha in 1976 to 1 335 000 ha in 2001. As part of the production system, field peas require inoculation with Rhizobium leguminosarum bv. Viceae, particularly, when grown for the first time in rotation. Appropriate rhizobial inoculation and fertility management can increase fie...

use of plant growth promoting bacteria (pgpb) and high quality of seed as agricultural inputs play important roles in sustainable crop production. to study the effects of different levels of seed aging and pgpb's application on field emergence, seedling establishment and yield of two red kidney bean cultivars in field conditions, an experiment was carried out as factorial based in a rcbd d...

In field peas, ascochyta blight is one of the most common fungal diseases caused by Didymella pinodes. Despite the high diversity of pea cultivars, only little resistance has been developed until to date, still leading to significant losses in grain yield. Rhizobia as plant growth promoting endosymbionts are the main partners for establishment of symbiosis with pea plants. The key role of Rhizo...