The maintenance of Leptospira species in liquid or semisolid medium is time-consuming and at risk of contamination due to the needs of routine subculture and dark field microscopy. Using Leptospira Vanaporn Wuthiekanun (LVW) agar, we maintained 100 pathogenic Leptospira isolates for 12 months without the need for subculture and confirmed the viability of all isolates by the naked eye.

A semisolid brucella medium with 10% sheep blood was used for storage and transport of Campylobacter fetus subsp. jejuni and subsp. intestinalis and kept isolated alive about 3 weeks or longer at 25 degrees C or when sent through the regular mail.

one of the most significant issues in pharmaceutical industries, prior to commercializationof a pharmaceutical preparation is the “preformulation” stage. however, far too attention hasbeen paid to verification of the software assisted statistical designs in preformulation studies.the main aim of this study was to report a step by step preformulation approach for a semisolidpreparation based on ...

BACKGROUND AND PURPOSE Acute-onset dysphagia after stroke is frequently associated with an increased risk of aspiration pneumonia. Because most screening tools are complex and biased toward fluid swallowing, we developed a simple, stepwise bedside screen that allows a graded rating with separate evaluations for nonfluid and fluid nutrition starting with nonfluid textures. The Gugging Swallowing...

Preliminary experiments revealed that coagulase positive staphylococci could be distinguished culturallv from coagulase negative staphylococci in a soft agar medium containing a small amount of normal human or rabbit plasma. In a medium of suitably low viscosity, staphylococci produce elongated diffuse feathery colonies. When coagulable plasma is incorporated in such a medium, the colonies of c...

Metal casting is an industrially important manufacturing process offering a superior combination of design flexibility, productivity and cost-effectiveness, but has limitations due to filling related defects. Several semisolid processes are available capable at range solid fractions overcome this. The current communication aims review the front behaviour give new perspective gate in processing ...

Current practice in designing tooling components for semisolid processing requires extensive commitments of human resources. Even when utilizing currently available Computer Aided Design (CAD) systems, tool design remains a complex task requiring large amount of design knowledge. Considerations such as part material, tool material, characteristics of die filling, flow behavior of semisolid slur...