Abstract: A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially us...

Bu çalışmada bazı ilaçların, koyun dalak dokusundan saflaştırılan glukoz-6-fosfat dehidrogenaz enzimi (G6PD; E.C. 1.1.1.49) üzerine in vitroetkileri araştırıldı. Çalışmanın ilk aşamasında G6PD salting out(amonyum sülfat çöktürmesi) metodu ve 2', 5' ADP-Sepharose 4B afinite kromatografisi ile saflaştırıldı enzimin saflık derecesi SDS-PAGE kontrol edildi. ikinci iveral, linkomisin, gentamisin, am...

There are a number of nuclear enzymes that bind nicotinamide, thymidine or nucleotides. We use this fact to prepare affinity columns for the purification of nuclear enzymes. In particular the nuclei of eukaryotic cells have an enzyme that catalyses the synthesis of the homopolymer poly(ADP-ribose) from NAD. Isolation and partial purification has been achieved (Yamada et al., 1971 ; Ueda et al.,...

The biological activity of Sepharose-insulin in different assays in vitro, e.g., stimulation of glucose oxidation, lipogenesis, and antilipolysis and activation of pyruvate dehydrogenase (EC 1.2.4.1) activity, has been investigated. According to amino acid analysis, between 270 and 330 mug (6.9-8.2 U) of insulin were coupled per ml of packed beads. Related to the total insulin content, 0.2-0.7%...

Diphtheria toxin fragment A interacts with Cibacron blue in solution, although it is not retained by blue Sepharose columns. Difference spectral titration of fragment A with the dye gives a dissociation constant of the order of 10(-5) M and a 1:1 stoichiometry for the complex. In equilibrium dialysis experiments Cibacron blue behaves as a competitive inhibitor of the binding of NAD to diphtheri...

Glycoprotein gp118, one of the major glycosylated proteins specified by varicella-zoster virus, is biologically of great importance since it possesses an epitope which elicits a complement-independent neutralizing antibody response. To purify this glycoprotein from a Nonidet-solubilized extract of varicella-zoster virus-infected cells, we examined its affinity to a variety of ligands, including...

Purification and PCR amplification procedures for DNA extracted from environmental samples (soil, compost, and river sediment) were improved by introducing three modifications: precipitation of DNA with 5% polyethylene glycol 8000 (PEG) and 0.6 M NaCl; filtration with a Sepharose 4B–polyvinylpolypyrrolidone (PVPP) spin column; and addition of skim milk (0.3% w/v) to the PCR reaction solution. H...

Myrosinase from white mustard seeds (Sinapis alba) has been purified starting from aqueous crude extract in a single step by affinity chromatography on Con A-Sepharose. The specific activity, recovery, and binding capacity in four separate trials using glucose, mannose, methyl a-D-glucoside, and methyl a-D-mannoside for elution were also determined. The enzyme isolated by our approach showed a ...

Twenty-eight 1-ethyland l-p ropyl-5-substitu ted imidazoles were synthesized and their bleaching activity was evaluated by using the lettuce seedling test. 5-Phenyl1-propylimidazole (1) caused distinct chlorosis, while none o f the com pounds with various alkenyl groups at the 5-position o f the im idazole ring showed this bleaching activity. In troduction o f a brom o, chloro, fluoro, methyl o...

Polysomes of HeLa cells were adsorbed on Poly(U)-Sepharose columns. This adsorption is probably due to poly(A) sequences at 3' terminus of the messenger. Stepwise elution initially removed ribosomal subunits thereafter mRNA and a set of proteins. These proteins are identical with the main components of the polysomal messenger ribonucleoprotein particles described previously. Thus, this method a...