In this study, the purposes were to compare the staining methods for detection of glycoprotein profiles in normal and lung cancer serum samples and to preliminary screen the carbohydrate specificity of glycoproteins in both serum samples. Using the sequential staining methods of Pro-Q Emerald 488 glycoprotein gel staining, SYPRO® Ruby staining and silver staining revealed that the Emerald 488 g...

Background: Oral spirochetes play a definitive role in the etiology of periodontitis. But their study is hampered due to difficulty in cultivating them and molecular methods are expensive. Hence the study explores the possibility of using staining as a screening procedure for the study of oral spirochetes. Experimental approach: The study included 100 patients with chronic periodontitis and 100...

Meiotic and mitotic chromosomes of Dichotomius nisus, D. semisquamosus and D. sericeus were analyzed after conventional staining, C-banding and silver nitrate staining. In addition, Dichotomius nisus and D. semisquamosus chromosomes were also analyzed after fluorescent in situ hybridization (FISH) with an rDNA probe. The species analyzed had an asymmetrical karyotype with 2n = 18 and meta-subme...

It has been recognized for some time that Mycobacterium leprae has the property of staining with the silver methods (2). However, there has not been described a simple, quick and easy technique for staining the bacillus in that way, applicable to either frozen or paraffin sections. It is my purpose to present a method which satisfies these requirements, and which also shows a new aspect of this...

Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.

A procedure for silver staining is described which leads to the selective and reliable impregnation of nerve fibers in bleached skin of vertebrates and invertebrates. In combination with osmium, the protocol enhances the staining of secondary sensory cells of mechanosensory and electrosensory organs so that the innervation pattern of each organ and the number of sensory cells per organ can easi...

A new simple modification to the silver staining of nucleolar organiser regions (AgNORs) was devised which, by performing the incubation with the slide inverted, results in minimal undesirable background staining, a persistent problem. Inverted incubation is facilitated by the use of a commercially available plastic coverplate. This technique has several additional advantages over other publish...