Single-channel recording provides high resolution information on gating mechanisms of ion channels that are generally difficult to obtain from macroscopic measurements. Analysis of the data, however, has proven to be challenging. Early approaches rely on half-amplitude threshold detection to idealize the record into dwell-times, followed by fitting duration histograms to resolve kinetics. More ...

The electrocardiography (ECG) artifact in surface electromyography (sEMG) is a major source of noise influencing the analyses. Moreover, in many cases the sEMG signal is the only available signal, making this removal more complicated. We compare the performance of two recently described single channel blind source separation methods with the commonly used template subtraction method on both sim...

This paper presents a new technique for achieving blind signal separation when given only a single channel recording. The main concept is based on exploiting a priori sets of time-domain basis functions learned by independent component analysis (ICA) to the separation of mixed source signals observed in a single channel. The inherent time structure of sound sources is reflected in the ICA basis...

White matter is a compact structure consisting primarily of neuronal axons and glial cells. As in other parts of the nervous system, the function of glial cells in white matter is poorly understood. We have explored the electrophysiological properties of two types of glial cells found predominantly in white matter: type 2 astrocytes and oligodendrocytes. Whole-cells and single-channel patch-cla...

The development of integral membrane protein cell-free synthesis permits in-vitro labeling of accessible cysteines for real-time FRET and LRET measurements. The functional integrity of these synthetic ion channel proteins has been verified at the whole oocyte level by direct injection into, and recording from, Xenopus oocytes. However, the microscopic single-channel properties of cell-free tran...

Since their refinement in the early 195Os, extracellular, singleunit recording methods have been used to obtain a wealth of data about the properties of CNS structures. The applications of the technique have been diverse: extracellular microelectrodes have been used to map the potential fields of single discharging neurons in order to answer fundamental questions about the excitability of CNS d...

In this paper we present results on single channel blind source separation based on a shift-invariant sparse coding model [1], [2] and [3]. This model learns a set of time-domain features from a single observation of the mixed signals. The found features can often be associated with a single source and can therefore be used to reconstruct the individual source signals. This is shown in this pap...

Patch fluorometry has emerged as a new approach to the study of the structure-function relationship in membrane-embedded functional ion channels. Simultaneous fluorescent and electrical recordings are achieved from a small number of channels in a cell-free membrane patch, yielding high recording sensitivities. Further improvement of this approach should permit direct observation of the gating m...

introduction: intracellular ph (phi) regulates essentially all aspects of cellular activities. however, it is unknown how endoplasmic reticulum (er) potassium channels sense phi. in this study, we investigate the direct effects of phi on er potassium channels. methods: we used channel incorporation into the bilayer lipid membrane method. l-α-phosphatidylcholine, a membrane lipid, was extracted ...