The main objective for plant breeding is to increase genetic diversity. Mutation induction is a method to increase genetic diversity associated with selection, recombination, or a combination of these approaches in plant breeding. The present research was aimed to compare efficacy of different doses of Gamma rays (150, 200, 250, 300 and 350 Gy and zero dose control) on the sesame morphological ...

The discovery of a single point mutation in the JAK2 gene in patients with BCR/ABL-negative myeloproliferative neoplasms (MPNs) has not only brought new insights and pathogenesis, but also has made the diagnosis of MPNs much easier. Although, to date, several mechanisms for the contribution of single JAK2V617F point mutation to phenotypic diversity of MPNs have been suggested in multiple studie...

An electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for point mutation detection has been developed. The target is amplified using a tris (bipyridine) ruthenium (TBR)-labeled forward and a biotinylated reverse primer. The amplification products are digested with specific restriction enzyme, then captured onto streptavidin-coated paramagnetic beads, and detected by measuring t...

AIM To develop a fast, low-cost diagnostic strategy to identify single point mutations in highly variable genomes such as hepatitis C virus (HCV). METHODS In patients with HCV infection, resistance-associated amino acid substitutions within the viral quasispecies prior to therapy can confer decreased susceptibility to direct-acting antiviral agents and lead to treatment failure and virologica...

conclusions in the present study, the frequency of clarithromycin resistance was lower than the other studies conducted in iran. resistance frequency in samples isolated from gastric ulcer was higher than other gastric disorders. women and patients aged more than 60 years old showed the most resistance frequency in this study. all resistant strains had the a2143g genotype. results of the 89 h. ...

High mutation rates typical of RNA viruses often generate a unique viral population structure consisting of a large number of genetic microvariants. In the case of viral pathogens, this can result in rapid evolution of antiviral resistance or vaccine-escape mutants. We determined a direct estimate of the mutation rate of measles virus, the next likely target for global elimination following pol...

Different mutation operators have been proposed in evolutionary programming, but for each operator there are some types of optimization problems that cannot be solved efficiently. A mixed strategy, integrating several mutation operators into a single algorithm, can overcome this problem. Inspired by evolutionary game theory, this paper presents a mixed strategy evolutionary programming algorith...

Macrolide treatment failure in syphilis patients is associated with a single point mutation (either A2058G or A2059G) in both copies of the 23S rRNA gene in Treponema pallidum strains. The conventional method for the detection of both point mutations uses nested PCR combined with restriction enzyme digestions, which is laborious and time-consuming. We initially developed a TaqMan-based real-tim...